MicroRNA Profiles of Maternal and Neonatal Endothelial Progenitor Cells in Preeclampsia
Abstract
:1. Introduction
2. Results
2.1. Demographic Characteristics
2.2. ECFC Isolation and Characterization
2.3. MiRNA Profiles in the Comparison Groups
2.3.1. MiRNA Differences between Preeclampsia and Healthy Pregnancies
2.3.2. MiRNA Differences between Maternal and Cord Blood
2.3.3. MiRNA Differences between Cell Culture Passages 3 and 5
2.4. Quantitative Real-Time PCR Validation and Putative Target Gene Levels
2.5. Pathway Enrichment Analysis
2.6. Hsa-miR-1270 Dependent Tube Formation, Proliferation and Chemotactic Motility
3. Discussion
4. Materials and Methods
4.1. Participants
4.2. ECFC Isolation and Characterization
4.3. Small RNA Sequencing
4.4. qRT-PCR for miRNA Validation and Putative Target Gene Expression
4.5. Transfection of ECFC with miRNA Inhibitor
4.6. Tube Formation Assay
4.7. Cell Proliferation Assay
4.8. Chemotaxis Assay
4.9. Statistics and Bioinformatics Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cord-Blood-Derived ECFC | Maternal-Blood-Derived ECFC | |||||
---|---|---|---|---|---|---|
Healthy Pregnancy (n = 6) | Preeclamptic Pregnancy (n = 6) | p-Value | Healthy Pregnancy (n = 6) | Preeclamptic Pregnancy (n = 6) | p-Value | |
Maternal age at delivery (years) | 32.8 ± 5.2 | 31.5 ± 3.7 | 0.65 | 31.7 ± 7.4 | 30.8 ± 5.5 | 0.85 |
Gestational age at delivery (weeks) | 38.6 ± 0.5 | 36.8 ± 1.1 | 0.03 | 38.3 ± 0.7 | 33.1 ± 3.9 | 0.01 |
Multiparous n (%) | 5 (83%) | 4 (67%) | 1.00 | 6 (100%) | 4 (67%) | 0.45 |
Maternal pre-pregnancy BMI (kg/m2) | 26.2 ± 6.3 | 29.7 ± 12.9 | 0.80 | 27.2 ± 4.5 | 26.9 ± 6.1 | 0.90 |
Gestational SBP, pre-delivery (mmHg) | 121 ± 8 | 164 ± 18 | <0.001 | 121 ± 8 | 163 ± 11 | <0.001 |
Gestational SBP, before 20 week gestation (mmHg) | 112 ± 11 | 125 ± 21 | 0.20 | 116 ± 14 | 113 ± 16 | 0.70 |
Gestational DBP, pre-delivery (mmHg) | 70 ± 5 | 98 ± 6 | <0.001 | 74 ± 9 | 95 ± 13 | 0.009 |
Gestational DBP, before 20 week gestation (mmHg) | 69 ± 11 | 80 ± 10 | 0.10 | 68 ± 11 | 76 ± 9 | 0.18 |
Birth weight (g) | 3487 ± 523 | 2741 ± 669 | 0.06 | 3314 ± 651 | 1628 ± 815 | 0.004 |
Birth weight percentile | 55 ± 30 | 35 ± 35 | 0.32 | 51 ± 35 | 21 ± 16 | 0.11 |
Birth weight percentile <10th n (%) | 0 (0%) | 1 (17%) | 1.00 | 1 (17%) | 2 (34%) | 1.00 |
Caesarean delivery n (%) | 6 (100%) | 5 (83%) | 1.00 | 6 (100%) | 6 (100%) | 1.00 |
Maternal race, White n (%) | 6 (100%) | 5 (83%) | 1.00 | 6 (100%) | 6 (100%) | 1.00 |
Baby sex, Male n (%) | 3 (50%) | 1 (17%) | 0.55 | 1 (17%) | 2 (34%) | 1.00 |
miRNA | Fold Change | p-Value |
---|---|---|
ECFC from Cord blood in P3 | ||
hsa-miR-4726-5p | −2.93 | 0.03 |
hsa-miR-1270 | −2.63 | 0.008 |
hsa-miR-148a-5p | −2.48 | 0.03 |
hsa-miR-1255a | −2.06 | 0.04 |
ECFC from Cord blood in P5 | ||
hsa-miR-2467-5p | 3.19 | 0.005 |
hsa-miR-4687-3p | 2.11 | 0.03 |
hsa-miR-148a-3p | −3.44 | 0.04 |
hsa-miR-1226-5p | −2.40 | 0.02 |
hsa-miR-3911 | −2.34 | 0.007 |
ECFC from Maternal blood in P3 | ||
hsa-miR-214-5p | −10.66 | 0.002 |
hsa-miR-199a-5p | −10.45 | 0.01 |
hsa-miR-214-3p | −8.89 | 0.005 |
hsa-miR-199a-3p | −8.52 | 0.005 |
hsa-miR-199b-3p | −8.52 | 0.005 |
hsa-miR-139-3p | −8.25 | 0.009 |
hsa-miR-139-5p | −4.13 | 0.04 |
hsa-miR-551a | −3.91 | 0.03 |
hsa-miR-4684-5p | −2.44 | 0.01 |
hsa-miR-3115 | −2.25 | 0.04 |
hsa-miR-3164 | −2.16 | 0.04 |
hsa-miR-199b-5p | −2.14 | 0.01 |
hsa-miR-4728-3 p | 5.06 | 0.003 |
hsa-miR-1250 | 3.10 | 0.04 |
hsa-miR-338-5p | 3.00 | 0.04 |
hsa-miR-338-3p | 2.92 | 0.04 |
hsa-miR-4485 | 2.75 | 0.03 |
hsa-miR-3177-5p | 2.27 | 0.01 |
hsa-miR-3128 | 2.21 | 0.04 |
hsa-miR-503 | 2.08 | 0.04 |
ECFC from Maternal blood in P5 | ||
hsa-miR-214-5p | −7.34 | 0.03 |
hsa-miR-214-3p | −6.70 | 0.02 |
hsa-miR-199a-3p | −6.26 | 0.04 |
hsa-miR-326 | −2.70 | 0.02 |
hsa-miR-491-3p | −2.09 | 0.04 |
hsa-miR-4511 | 3.45 | 0.007 |
hsa-miR-3128 | 3.18 | 0.002 |
hsa-miR-4421 | 2.41 | 0.04 |
hsa-miR-2682-5p | 2.23 | 0.02 |
hsa-miR-3177-5p | 2.19 | 0.001 |
hsa-miR-5690 | 2.08 | 0.03 |
hsa-miR-2277-3p | 2.06 | 0.04 |
miRNA | Fold Change | p-Value |
---|---|---|
Cord blood ECFC from controls in P3 vs. P5 | ||
hsa-miR-4725-3p | −2.83 | 0.0007 |
hsa-miR-1293 | −2.44 | 0.04 |
hsa-miR-3155a | −2.30 | 0.03 |
hsa-miR-548s | −2.19 | 0.03 |
hsa-miR-3911 | −2.16 | 0.007 |
hsa-miR-451a | 3.70 | 0.007 |
hsa-miR-122-5p | 3.20 | 0.004 |
hsa-miR-5582-3p | 2.36 | 0.03 |
hsa-miR-4726-5p | 2.14 | 0.04 |
hsa-miR-2682-3p | 2.10 | 0.03 |
Cord blood ECFC from preeclampsia in P3 vs. P5 | ||
hsa-miR-1226-5p | −28.23 | 0.008 |
hsa-miR-5001-5p | −25.60 | 0.02 |
hsa-miR-3911 | −21.26 | 0.02 |
hsa-miR-3149 | 20.86 | 0.008 |
hsa-miR-4791 | 20.16 | 0.04 |
hsa-miR-2467-5p | 3.77 | 0.002 |
Maternal blood ECFC from controls in P3 vs. P5 | ||
hsa-let-7g-3p | −24.30 | 0.008 |
hsa-miR-4421 | −2.97 | 0.003 |
Maternal blood ECFC from preeclampsia in P3 vs. P5 | ||
hsa-miR-4677-5p | −2.45 | 0.03 |
hsa-miR-4754 | 2.22 | 0.01 |
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Brodowski, L.; Schröder-Heurich, B.; von Hardenberg, S.; Richter, K.; von Kaisenberg, C.S.; Dittrich-Breiholz, O.; Meyer, N.; Dörk, T.; von Versen-Höynck, F. MicroRNA Profiles of Maternal and Neonatal Endothelial Progenitor Cells in Preeclampsia. Int. J. Mol. Sci. 2021, 22, 5320. https://doi.org/10.3390/ijms22105320
Brodowski L, Schröder-Heurich B, von Hardenberg S, Richter K, von Kaisenberg CS, Dittrich-Breiholz O, Meyer N, Dörk T, von Versen-Höynck F. MicroRNA Profiles of Maternal and Neonatal Endothelial Progenitor Cells in Preeclampsia. International Journal of Molecular Sciences. 2021; 22(10):5320. https://doi.org/10.3390/ijms22105320
Chicago/Turabian StyleBrodowski, Lars, Bianca Schröder-Heurich, Sandra von Hardenberg, Katja Richter, Constantin S. von Kaisenberg, Oliver Dittrich-Breiholz, Nadia Meyer, Thilo Dörk, and Frauke von Versen-Höynck. 2021. "MicroRNA Profiles of Maternal and Neonatal Endothelial Progenitor Cells in Preeclampsia" International Journal of Molecular Sciences 22, no. 10: 5320. https://doi.org/10.3390/ijms22105320