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Volume 22
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10.3390/ijms22105327
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Article

Constitutive Expression of TERT Enhances β-Klotho Expression and Improves Age-Related Deterioration in Early Bovine Embryos

by
Lianguang Xu
1,†,
Muhammad Idrees
1,2,†,
Myeong-Don Joo
1,
Tabinda Sidrat
1,
Yiran Wei
1,
Seok-Hwan Song
3,
Kyeong-Lim Lee
3 and
Il-Keun Kong
1,2,3,*
1
Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Gyeongnam Province, Korea
2
Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Gyeongnam Province, Korea
3
The Kingkong Co., Ltd., Gyeongsang National University, Jinju 52828, Gyeongnam Province, Korea
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2021, 22(10), 5327; https://doi.org/10.3390/ijms22105327
Submission received: 22 March 2021 / Revised: 14 May 2021 / Accepted: 17 May 2021 / Published: 18 May 2021
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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Abstract

Age-associated decline in oocyte quality is one of the dominant factors of low fertility. Aging alters several key processes, such as telomere lengthening, cell senescence, and cellular longevity of granulosa cells surrounding oocyte. To investigate the age-dependent molecular changes, we examined the expression, localization, and correlation of telomerase reverse transcriptase (TERT) and β-Klotho (KLB) in bovine granulosa cells, oocytes, and early embryos during the aging process. Herein, cumulus-oocyte complexes (COCs) obtained from aged cows (>120 months) via ovum pick-up (OPU) showed reduced expression of β-Klotho and its co-receptor fibroblast growth factor receptor 1 (FGFR1). TERT plasmid injection into pronuclear zygotes not only markedly enhanced day-8 blastocysts’ development competence (39.1 ± 0.8%) compared to the control (31.1 ± 0.5%) and D-galactose (17.9 ± 1.0%) treatment groups but also enhanced KLB and FGFR1 expression. In addition, plasmid-injected zygotes displayed a considerable enhancement in blastocyst quality and implantation potential. Cycloastragenol (CAG), an extract of saponins, stimulates telomerase enzymes and enhances KLB expression and alleviates age-related deterioration in cultured primary bovine granulosa cells. In conclusion, telomerase activation or constitutive expression will increase KLB expression and activate the FGFR1/β-Klotho pathway in bovine granulosa cells and early embryos, inhibiting age-related malfunctioning.
Keywords: β-Klotho; FGFR1; TERT; cycloastragenol; bovine embryo β-Klotho; FGFR1; TERT; cycloastragenol; bovine embryo
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MDPI and ACS Style

Xu, L.; Idrees, M.; Joo, M.-D.; Sidrat, T.; Wei, Y.; Song, S.-H.; Lee, K.-L.; Kong, I.-K. Constitutive Expression of TERT Enhances β-Klotho Expression and Improves Age-Related Deterioration in Early Bovine Embryos. Int. J. Mol. Sci. 2021, 22, 5327. https://doi.org/10.3390/ijms22105327

AMA Style

Xu L, Idrees M, Joo M-D, Sidrat T, Wei Y, Song S-H, Lee K-L, Kong I-K. Constitutive Expression of TERT Enhances β-Klotho Expression and Improves Age-Related Deterioration in Early Bovine Embryos. International Journal of Molecular Sciences. 2021; 22(10):5327. https://doi.org/10.3390/ijms22105327

Chicago/Turabian Style

Xu, Lianguang, Muhammad Idrees, Myeong-Don Joo, Tabinda Sidrat, Yiran Wei, Seok-Hwan Song, Kyeong-Lim Lee, and Il-Keun Kong. 2021. "Constitutive Expression of TERT Enhances β-Klotho Expression and Improves Age-Related Deterioration in Early Bovine Embryos" International Journal of Molecular Sciences 22, no. 10: 5327. https://doi.org/10.3390/ijms22105327

APA Style

Xu, L., Idrees, M., Joo, M.-D., Sidrat, T., Wei, Y., Song, S.-H., Lee, K.-L., & Kong, I.-K. (2021). Constitutive Expression of TERT Enhances β-Klotho Expression and Improves Age-Related Deterioration in Early Bovine Embryos. International Journal of Molecular Sciences, 22(10), 5327. https://doi.org/10.3390/ijms22105327

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