MAT2A Localization and Its Independently Prognostic Relevance in Breast Cancer Patients
Abstract
:1. Introduction
2. Results
2.1. Identification of mRNA Expression of the Methionine Cycle Genes Signature for Survival in TGCA Dataset
2.2. Expression of GNMT Is Downregulated, MAT1A Is Upregulated, and Nuclear MAT2A Is Downregulated in Breast Cancer Tissues
2.3. Identification of a Gene Expression Signature for Survival
2.4. Localization of MAT2A in Breast Cancer Cell Lines and the Association of MAT2A C/N Ratio and Cell Invasiveness
3. Discussion
4. Materials and Methods
4.1. Web Server Survival Analysis
4.2. Patients
4.3. Immunohistochemistry and Scoring
4.4. Cell Culture
4.5. Western Blot Analysis
4.6. Transwell Invasion Assay
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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GNMT1 | MAT1A | MAT2A (C/N Ratio) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Characteristics | No. | Low (N = 126) | High (N = 126) | p-Value | Low (N = 126) | High (N = 126) | p-Value | Low (N = 126) | High (N = 126) | p-Value |
Age | ||||||||||
<65 | 206 | 99 (48) | 107 (52) | 0.19 | 97 (47) | 109 (53) | 0.05 | 109 (53) | 97 (47) | 0.05 |
≥65 | 46 | 27 (59) | 19 (41) | 29 (63) | 17 (37) | 17 (37) | 29 (63) | |||
Stage | ||||||||||
I, II | 183 | 52 (28) | 131 (72) | 0.89 | 72 (39) | 111 (61) | 0.81 | 95 (52) | 88 (48) | 0.323 |
III, IV | 69 | 19 (28) | 50 (72) | 26 (38) | 43 (62) | 31 (45) | 38 (55) | |||
ER | ||||||||||
Negative | 71 | 37 (52) | 34 (48) | 0.67 | 34 (48) | 47 (52) | 0.67 | 37 (52) | 34 (48) | 0.674 |
Positive | 181 | 89 (49) | 92 (51) | 92 (51) | 89 (49) | 89 (49) | 92 (51) | |||
PR | ||||||||||
Negative | 98 | 54 (55) | 44 (45) | 0.2 | 50 (51) | 48 (49) | 0.8 | 49 (50) | 49 (50) | 1 |
Positive | 154 | 72 (47) | 82 (53) | 76 (49) | 78 (51) | 77 (50) | 77 (50) | |||
HER2 | ||||||||||
Negative | 170 | 82 (48) | 88 (52) | 0.42 | 84 (49) | 86 (51) | 0.79 | 89 (52) | 81 (48) | 0.282 |
Positive | 82 | 44 (54) | 38 (46) | 42 (51) | 40 (49) | 37 (45) | 45 (55) |
Characteristics | (Favorable/Unfavorable) | HR | 95.0% CI | p-Value |
---|---|---|---|---|
Age | <65/≥65 | 3.730 | 1.717–8.101 | 0.004 |
ER | Negative/Positive | 4.442 | 2.002–9.855 | <0.001 |
Stage | I, II/III, IV | 8.276 | 3.627–18.884 | <0.001 |
MAT2A (C/N ratio) | Low/High | 2.771 | 1.186–6.472 | 0.018 |
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Chu, P.-Y.; Wu, H.-J.; Wang, S.-M.; Chen, P.-M.; Tang, F.-Y.; Chiang, E.-P.I. MAT2A Localization and Its Independently Prognostic Relevance in Breast Cancer Patients. Int. J. Mol. Sci. 2021, 22, 5382. https://doi.org/10.3390/ijms22105382
Chu P-Y, Wu H-J, Wang S-M, Chen P-M, Tang F-Y, Chiang E-PI. MAT2A Localization and Its Independently Prognostic Relevance in Breast Cancer Patients. International Journal of Molecular Sciences. 2021; 22(10):5382. https://doi.org/10.3390/ijms22105382
Chicago/Turabian StyleChu, Pei-Yi, Hsing-Ju Wu, Shin-Mae Wang, Po-Ming Chen, Feng-Yao Tang, and En-Pei Isabel Chiang. 2021. "MAT2A Localization and Its Independently Prognostic Relevance in Breast Cancer Patients" International Journal of Molecular Sciences 22, no. 10: 5382. https://doi.org/10.3390/ijms22105382