Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons
Abstract
:1. Introduction
2. Results
2.1. Analysis of VAChT and ChAT Splice Variants
2.2. ChAT Protein Expression
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. RNA Extraction
4.3. RT-PCR and qRT- PCR Analysis
4.3.1. RT–PCR Analysis
4.3.2. qRT- PCR Analysis
4.3.3. Primers
4.4. Protein Extraction and Western Blot Analysis
4.5. ChAT Assay
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Acknowledgments
Conflicts of Interest
Abbreviations
Ach | Acetylcholine |
cAMP | Cyclic adenosine monophosphate |
ChAT | Choline acetyltransferase |
CGRP | Calcitonin gene related peptide |
CNTF | Ciliary neurotrophic factor |
DRG | Dorsal root ganglion |
FBS | Fetal bovine serum |
GAPDH | Glyceraldehyde phosphodehydrogenase |
HPRT | Hypoxanthine phosphoribosyl transferase |
NG | Neuroblastoma-glioma |
NGF | Nerve growth factor |
RA | Retinoic acid |
SDS | Sodium dodecyl sulphate |
VAChT | Vesicular acetylcholine transporter |
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Corsetti, V.; Perrone-Capano, C.; Salazar Intriago, M.S.; Botticelli, E.; Poiana, G.; Augusti-Tocco, G.; Biagioni, S.; Tata, A.M. Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons. Int. J. Mol. Sci. 2021, 22, 5499. https://doi.org/10.3390/ijms22115499
Corsetti V, Perrone-Capano C, Salazar Intriago MS, Botticelli E, Poiana G, Augusti-Tocco G, Biagioni S, Tata AM. Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons. International Journal of Molecular Sciences. 2021; 22(11):5499. https://doi.org/10.3390/ijms22115499
Chicago/Turabian StyleCorsetti, Veronica, Carla Perrone-Capano, Michael Sebastian Salazar Intriago, Elisabetta Botticelli, Giancarlo Poiana, Gabriella Augusti-Tocco, Stefano Biagioni, and Ada Maria Tata. 2021. "Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons" International Journal of Molecular Sciences 22, no. 11: 5499. https://doi.org/10.3390/ijms22115499