Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition
Abstract
:1. Introduction
2. Results
2.1. Mulberry miR156 Is Differentially Expressed in Juvenile and Mature Phase
2.2. Identification of the miR156 Target Genes in Mulberry
2.3. MnSPLs Bound to the Promoter of mno-miR172a and Promoted Its Expression in Mulberry
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Bioinformatics Analysis of miR156, SPL Genes, and miR172 in Mulberry
4.3. Epression Analysis of miRNAs and Genes
4.4. Transient Trnsgene Expression in Mulberry
4.5. Dual-Luciferase Repoter Assay
4.6. Yeast One-Hybrid Assay
4.7. Degradome Sequencing
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Sequence | Length | Read in Leaf | Read in Bark | Read in Male Flower |
---|---|---|---|---|---|
mno-miR156a | GCTCACTTCTCTTTCTGTCAGC | 22 | 0 | 46 | 4 |
mno-miR156b | TTGACAGAAGAGAGAGAGCACA | 22 | 6 | 2 | 337 |
mno-miR156c | TTGACAGAAGATAGAGAGCAC | 21 | 12,787 | 117,270 | 768,488 |
mno-miR156d | TTGACAGAAGAGAGTGAGCAC | 21 | 2942 | 14,574 | 8723 |
mno-miR156e | TTGACAGAAGAGAGAGAGCAC | 21 | 2099 | 1277 | 155,199 |
mno-miR156f | TGACAGAAGAGAGTGAGCACA | 21 | 22 | 378 | 65 |
mno-miR156g | TGACAGAAGAGAGAGAGCACA | 21 | 8 | 4 | 1198 |
mno-miR172a | AGAATCTTGATGATGCTGCAT | 21 | 19,265 | 8484 | 1245 |
mno-miR172b | AGAATCTTGATGATGCTGCAG | 21 | 143 | 13 | 137 |
mno-miR172c | GGAATCTTGATGATGCTGCAT | 21 | 15 | 266 | 6 |
mno-miR172d | GGAGCATCATCAAGATTCACA | 21 | 11 | 6 | 4 |
mno-miR172e | AGAATCCTGATGATGCTGCAT | 21 | 3 | 2 | 0 |
mno-miR172f | GTAGCATCATCAAGATTCACA | 21 | 1 | 6 | 0 |
Target Acc. | Expectation | Target Start | Target End | Alignment | Target Aligned Fragment | Inhibition | Molecular Function (Go) | Gene Family |
---|---|---|---|---|---|---|---|---|
Morus010792 | 0 | 1554 | 1574 | ::::::::::::::::::::: | GUGCUCUCUAUCUUCUGUCAA | Cleavage | Binding | SPL |
Morus010123 | 1 | 2201 | 2221 | :::::::::::::::::::: | GUGCUCUCUAUCUUCUGUCAU | Cleavage | Binding | SPL |
Morus015493 | 1.5 | 2135 | 2155 | ::::::::: ::::::::::: | GUGCUCUCUCUCUUCUGUCAA | Cleavage | Binding | SPL |
Morus018032 | 1.5 | 2589 | 2609 | ::::::::: ::::::::::: | GUGCUCUCUCUCUUCUGUCAA | Cleavage | Binding | SPL |
Morus014488 | 1.5 | 2018 | 2038 | :::::::: ::::::::::: | UUGCUCUCUCUCUUCUGUCAA | Cleavage | Binding | SPL |
Morus021290 | 2 | 1586 | 1606 | : .:::::::::::::::.: | UUUUUCUCUAUCUUCUGUCGA | Cleavage | / | / |
Morus017456 | 2.5 | 1220 | 1240 | ::::::::: :::::::::: | GUGCUCUCUCUCUUCUGUCAU | Cleavage | Binding | SPL |
Morus026457 | 2.5 | 2801 | 2821 | ::::::::: :::::::::: | GUGCUCUCUCUCUUCUGUCAU | Cleavage | Binding | SPL |
Morus026689 | 2.5 | 549 | 569 | :::::.:.:::.::::::.:. | GUGCUUUUUAUUUUCUGUUAG | Cleavage | Binding | WD40 repeat-like |
Morus002371 | 2.5 | 3369 | 3389 | :::::.:::::.:::: :::: | GUGCUUUCUAUUUUCUAUCAA | Cleavage | Binding | WD40 repeat-like |
Morus011698 | 2.5 | 3694 | 3714 | : :.:.:::.::::::::..: | GAGUUUUCUGUCUUCUGUUGA | Cleavage | Binding | WD40 repeat-like |
Morus017876 | 2.5 | 3883 | 3903 | :.:.:::.:.::::::.:: | UAGUUUUCUGUUUUCUGUUAA | Cleavage | catalytic activity | FBP |
Morus006566 | 2.5 | 1116 | 1136 | :.:: .::::::.:::::: | CCGUUCAUUAUCUUUUGUCAA | Cleavage | / | / |
Gene_ID1 | Gene_ID2 | Gene Name | T_Start | T_Stop | T_Slice | D_RawReads | D_Pval |
---|---|---|---|---|---|---|---|
Morus010123 | XM_010088675.2 | MnSPL13 | 1221 | 1241 | 1232 | 25 | 0.00 |
Morus010792 | XM_010114085.2 | MnSPL16A | 1078 | 1098 | 1089 | 875 | 0.00 |
Morus015493 | XM_024169654.1 | MnSPL2 | 1452 | 1472 | 1463 | 68 | 0.00 |
Morus018032 | XM_010104258.2 | MnSPL15 | 1129 | 1149 | 1140 | 142 | 0.00 |
Morus026457 | XM_024175201.1 | MnSPL6 | 1580 | 1600 | 1591 | 151 | 0.00 |
Morus017456 | XM_024173253.1 | MnSPL16B | 758 | 778 | 769 | 140 | 0.01 |
Morus014488 | XM_010109861.2 | MnSPL4 | 842 | 862 | 853 | 26 | 0.01 |
Morus018550 | XM_024174390.1 | MnGR-RBP7 | 587 | 607 | 598 | 6 | 0.36 |
Morus012399 | XM_010089645.2 | MnrsmG | 171 | 191 | 182 | 1 | 0.87 |
Morus014475 | XM_024170153.1 | MnRH13 | 1613 | 1640 | 1631 | 1 | 0.97 |
Morus021827 | XM_024177195.1 | MnCABP5 | 446 | 464 | 455 | 1 | 0.99 |
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Li, H.; Luo, Y.; Ma, B.; Hu, J.; Lv, Z.; Wei, W.; Hao, H.; Yuan, J.; He, N. Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition. Int. J. Mol. Sci. 2021, 22, 5550. https://doi.org/10.3390/ijms22115550
Li H, Luo Y, Ma B, Hu J, Lv Z, Wei W, Hao H, Yuan J, He N. Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition. International Journal of Molecular Sciences. 2021; 22(11):5550. https://doi.org/10.3390/ijms22115550
Chicago/Turabian StyleLi, Hongshun, Yiwei Luo, Bi Ma, Jianqiong Hu, Zhiyuan Lv, Wuqi Wei, Haiye Hao, Jianglian Yuan, and Ningjia He. 2021. "Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition" International Journal of Molecular Sciences 22, no. 11: 5550. https://doi.org/10.3390/ijms22115550