Apex Resection in Zebrafish (Danio rerio) as a Model of Heart Regeneration: A Video-Assisted Guide
Abstract
:1. Introduction
2. Results
2.1. Finetuning Anaesthesia in Zebrafish
2.2. Apex Resection (AR) in Zebrafish Step-by-Step
2.3. 2D- and 3D-Analysis for Heart Regeneration
3. Discussion
4. Materials and Methods
4.1. Ethics
4.2. Zebrafish
4.3. Materials Required for Apex Resection (AR) in Zebrafish
- 2 small aquariums/tanks for sedation and recovery
- Small fishing net
- Microscope (e.g., Leica, S9i)
- Large plastic spoon
- Pasteur pipette
- Fine curved forceps (S&T, Agnthos, Lidingo, Sweden, cat. no. JFCL-3P TC)
- Micro scissors (LAWTON, Fridingen an der Donau, Germany, cat. no. 05-0050-C)
- Sponge with pre-cut slit big enough to hold the zebrafish
- Pro-ophta, pre-cut on the middle in a 30° angle/cotton swab
- Tissues for removing (zebrafish) scales from surgical instruments
- Tricaine solutions in water from your system/aquarium for anaesthesia
- Water from your system/aquarium
4.4. Tricaine Stock Solution
4.5. Anaesthesia of Zebrafish
4.6. Surgical Procedures and Recovery
4.7. Tissue Processing for 2D Analysis
4.8. Haematoxylin and Eosin Staining
4.9. Masson’s Trichrome Staining
4.10. Fluorescence staining with Myosin Heavy Chain Antibody (MYH1), Wheat Germ Agglutinin (WGA) and 4′,6′-diamidino-2-phenylindole (DAPI)
4.11. CUBIC Tissue Clearing and 2-Photon Imaging
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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Ellman, D.G.; Slaiman, I.M.; Mathiesen, S.B.; Andersen, K.S.; Hofmeister, W.; Ober, E.A.; Andersen, D.C. Apex Resection in Zebrafish (Danio rerio) as a Model of Heart Regeneration: A Video-Assisted Guide. Int. J. Mol. Sci. 2021, 22, 5865. https://doi.org/10.3390/ijms22115865
Ellman DG, Slaiman IM, Mathiesen SB, Andersen KS, Hofmeister W, Ober EA, Andersen DC. Apex Resection in Zebrafish (Danio rerio) as a Model of Heart Regeneration: A Video-Assisted Guide. International Journal of Molecular Sciences. 2021; 22(11):5865. https://doi.org/10.3390/ijms22115865
Chicago/Turabian StyleEllman, Ditte Gry, Ibrahim Mohamad Slaiman, Sabrina Bech Mathiesen, Kristian Skriver Andersen, Wolfgang Hofmeister, Elke Annette Ober, and Ditte Caroline Andersen. 2021. "Apex Resection in Zebrafish (Danio rerio) as a Model of Heart Regeneration: A Video-Assisted Guide" International Journal of Molecular Sciences 22, no. 11: 5865. https://doi.org/10.3390/ijms22115865