Magnesium Isoglycyrrhizinate Reduces Hepatic Lipotoxicity through Regulating Metabolic Abnormalities
Abstract
:1. Introduction
2. Results
2.1. The Protective Effect of MGIG on PA-INDUCED Cytotoxicity
2.2. The Protective Effect of MGIG against PA-Induced Organelle Damage
2.3. The Regulatory Effect of MGIG on PA-Induced Metabolic Reprogramming
2.4. The Regulatory Effect of MGIG on PA-Induced Abnormal Lipid Metabolism
3. Discussion and Conclusions
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Cell Culture
4.3. Cell Viability Assay
4.4. Cell Proliferation Assay
4.5. Cell Damage Assay
4.6. Gene Expression Profiling and qPCR
4.7. Metabolomics Analysis
4.7.1. Metabolomics Sample Preparation
4.7.2. Metabolomics Sample Detection
4.7.3. Metabolomics Data Analysis
4.8. Lipidomics Analysis
4.8.1. Lipidomics Sample Preparation
4.8.2. Lipidomics Sample Detection
4.8.3. Lipidomics Data Analysis
4.9. Cytoscape Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Lu, L.; Hao, K.; Hong, Y.; Liu, J.; Zhu, J.; Jiang, W.; Zhu, Z.; Wang, G.; Peng, Y. Magnesium Isoglycyrrhizinate Reduces Hepatic Lipotoxicity through Regulating Metabolic Abnormalities. Int. J. Mol. Sci. 2021, 22, 5884. https://doi.org/10.3390/ijms22115884
Lu L, Hao K, Hong Y, Liu J, Zhu J, Jiang W, Zhu Z, Wang G, Peng Y. Magnesium Isoglycyrrhizinate Reduces Hepatic Lipotoxicity through Regulating Metabolic Abnormalities. International Journal of Molecular Sciences. 2021; 22(11):5884. https://doi.org/10.3390/ijms22115884
Chicago/Turabian StyleLu, Li, Kun Hao, Yu Hong, Jie Liu, Jinwei Zhu, Wenjiao Jiang, Zheying Zhu, Guangji Wang, and Ying Peng. 2021. "Magnesium Isoglycyrrhizinate Reduces Hepatic Lipotoxicity through Regulating Metabolic Abnormalities" International Journal of Molecular Sciences 22, no. 11: 5884. https://doi.org/10.3390/ijms22115884