Orphan Nuclear Receptor ERRγ Is a Transcriptional Regulator of CB1 Receptor-Mediated TFR2 Gene Expression in Hepatocytes
Abstract
:1. Introduction
2. Results
2.1. CB1 Receptor Signaling Increases ERRγ and TFR2 Gene Expression in Hepatocytes
2.2. ERRγ Increases TFR2 Gene Expression
2.3. Knockdown of ERRγ Decreases CB1 Receptor-Mediated TFR2 Expression
2.4. TFR2 Is a Direct Target of ERRγ
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Plasmid DNAs and Recombinant Adenoviruses
4.3. Cell Culture, Transfection and Luciferase Assay
4.4. Animal Experiment
4.5. Quantitative Real-Time PCR Analysis
4.6. Protein Extraction and Western Blot Analysis
4.7. Chromatin Immunoprecipitation (ChIP) Assay
4.8. RNA Interference
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, B.-E.; Choi, B.; Park, W.-R.; Kim, Y.-J.; Kim, I.-Y.; Jung, Y.S.; Kim, Y.-H.; Lee, C.-H.; Choi, H.-S.; Kim, D.-K. Orphan Nuclear Receptor ERRγ Is a Transcriptional Regulator of CB1 Receptor-Mediated TFR2 Gene Expression in Hepatocytes. Int. J. Mol. Sci. 2021, 22, 6021. https://doi.org/10.3390/ijms22116021
Kim B-E, Choi B, Park W-R, Kim Y-J, Kim I-Y, Jung YS, Kim Y-H, Lee C-H, Choi H-S, Kim D-K. Orphan Nuclear Receptor ERRγ Is a Transcriptional Regulator of CB1 Receptor-Mediated TFR2 Gene Expression in Hepatocytes. International Journal of Molecular Sciences. 2021; 22(11):6021. https://doi.org/10.3390/ijms22116021
Chicago/Turabian StyleKim, Bo-Eun, Byungyoon Choi, Woo-Ram Park, Yu-Ji Kim, In-Young Kim, Yoon Seok Jung, Yong-Hoon Kim, Chul-Ho Lee, Hueng-Sik Choi, and Don-Kyu Kim. 2021. "Orphan Nuclear Receptor ERRγ Is a Transcriptional Regulator of CB1 Receptor-Mediated TFR2 Gene Expression in Hepatocytes" International Journal of Molecular Sciences 22, no. 11: 6021. https://doi.org/10.3390/ijms22116021