In Vitro Characterization of Guanylyl Cyclase BdPepR2 from Brachypodium distachyon Identified through a Motif-Based Approach
Abstract
:1. Introduction
2. Results and Discussion
2.1. Sequence Analysis of BdPepR2
2.2. Purification of BdPepR2 Recombinant Protein and In Vitro GC Activity
2.3. Mutational Analysis
2.4. BdPepR2 Kinase Activity
3. Materials and Methods
3.1. Construction of Expression Vectors
3.2. Expression and Purification of the Recombinant Proteins
3.3. Determination of Guanylyl Cyclase Activity
3.4. Determination of Proteins Kinase Activity
3.5. Computational Modeling
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Duszyn, M.; Świeżawska-Boniecka, B.; Wong, A.; Jaworski, K.; Szmidt-Jaworska, A. In Vitro Characterization of Guanylyl Cyclase BdPepR2 from Brachypodium distachyon Identified through a Motif-Based Approach. Int. J. Mol. Sci. 2021, 22, 6243. https://doi.org/10.3390/ijms22126243
Duszyn M, Świeżawska-Boniecka B, Wong A, Jaworski K, Szmidt-Jaworska A. In Vitro Characterization of Guanylyl Cyclase BdPepR2 from Brachypodium distachyon Identified through a Motif-Based Approach. International Journal of Molecular Sciences. 2021; 22(12):6243. https://doi.org/10.3390/ijms22126243
Chicago/Turabian StyleDuszyn, Maria, Brygida Świeżawska-Boniecka, Aloysius Wong, Krzysztof Jaworski, and Adriana Szmidt-Jaworska. 2021. "In Vitro Characterization of Guanylyl Cyclase BdPepR2 from Brachypodium distachyon Identified through a Motif-Based Approach" International Journal of Molecular Sciences 22, no. 12: 6243. https://doi.org/10.3390/ijms22126243