In Vitro Evaluation of the Individual and Combined Cytotoxic and Estrogenic Effects of Zearalenone, Its Reduced Metabolites, Alternariol, and Genistein
Abstract
:1. Introduction
2. Results and Discussion
2.1. Individual Effects of ZEN, ZEN Metabolites, and AOH on Cell Viability
2.2. Combined Effects of Mycotoxins on Cell Viability
2.3. Individual Estrogenic Effects of ZEN, ZEN Metabolites, AOH, and GEN
2.4. Combined Estrogenic Effects of ZEN, ZEN Metabolites, AOH, and GEN
3. Materials and Methods
3.1. Reagents
3.2. Cell Culturing, Treatment, and Cell Viability Assay
3.3. BLYES Assay in Saccharomyces Cerevisiae
3.4. Assessment of Combined Effects
3.5. Statistics
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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20 µM ZEN + | CI Value | Combined Cytotoxic Effect | |
---|---|---|---|
10 µM α-ZEL | 0.57 | Synergism | ++ |
30 µM α-ZEL | 0.81 | Additive effect | ± |
10 µM β-ZEL | 0.64 | Synergism | + |
30 µM β-ZEL | 1.07 | Additive effect | ± |
10 µM ZAN | 0.55 | Synergism | ++ |
30 µM ZAN | 0.77 | Synergism | + |
10 µM α-ZAL | 0.52 | Synergism | ++ |
30 µM α-ZAL | 0.78 | Synergism | + |
10 µM β-ZAL | 0.51 | Synergism | ++ |
30 µM β-ZAL | 0.72 | Synergism | + |
10 µM AOH | 0.73 | Synergism | + |
30 µM AOH | 0.50 | Synergism | ++ |
6.28 × 10−2 µM ZEN + | CI Value | Combined Estrogenic Effect | |
---|---|---|---|
6.24 × 10−3 µM α-ZEL | 0.37 | Synergism | ++ |
1.25 × 10−2 µM α-ZEL | 0.41 | Synergism | ++ |
1.22 µM β-ZEL | 0.77 | Synergism | + |
2.43 µM β-ZEL | 0.95 | Additive effect | ± |
2.50 × 10−2 µM ZAN | 0.58 | Synergism | ++ |
4.99 × 10−2 µM ZAN | 0.62 | Synergism | + |
1.24 × 10−2 µM α-ZAL | 0.97 | Additive effect | ± |
2.48 × 10−2 µM α-ZAL | 1.16 | Additive effect | ± |
1.24 × 10−1 µM β-ZAL | 1.19 | Additive effect | ± |
2.48 × 10−1 µM β-ZAL | 1.56 | Antagonism | -- |
7.4 × 10−1 µM GEN | 0.58 | Synergism | ++ |
1.48 µM GEN | 0.77 | Synergism | + |
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Balázs, A.; Faisal, Z.; Csepregi, R.; Kőszegi, T.; Kriszt, B.; Szabó, I.; Poór, M. In Vitro Evaluation of the Individual and Combined Cytotoxic and Estrogenic Effects of Zearalenone, Its Reduced Metabolites, Alternariol, and Genistein. Int. J. Mol. Sci. 2021, 22, 6281. https://doi.org/10.3390/ijms22126281
Balázs A, Faisal Z, Csepregi R, Kőszegi T, Kriszt B, Szabó I, Poór M. In Vitro Evaluation of the Individual and Combined Cytotoxic and Estrogenic Effects of Zearalenone, Its Reduced Metabolites, Alternariol, and Genistein. International Journal of Molecular Sciences. 2021; 22(12):6281. https://doi.org/10.3390/ijms22126281
Chicago/Turabian StyleBalázs, Adrienn, Zelma Faisal, Rita Csepregi, Tamás Kőszegi, Balázs Kriszt, István Szabó, and Miklós Poór. 2021. "In Vitro Evaluation of the Individual and Combined Cytotoxic and Estrogenic Effects of Zearalenone, Its Reduced Metabolites, Alternariol, and Genistein" International Journal of Molecular Sciences 22, no. 12: 6281. https://doi.org/10.3390/ijms22126281