Toxicity and Antioxidant Activity of Fullerenol C60,70 with Low Number of Oxygen Substituents
Abstract
:1. Introduction
2. Results and Discussion
2.1. Fullerenol Toxicity
2.2. Fullerenol Antioxidant Activity
2.2.1. Change of General Toxicity under Conditions of Oxidative Exposure
2.2.2. Change of Oxidative Toxicity under Conditions of Oxidative Exposure
3. Materials and Methods
3.1. Preparation and Characterization of Fullerenols
3.2. Bioluminescence Enzymatic Assay and Experimental Data Processing
3.3. Luminol Chemiluminescence Assay
3.4. Statistical Processing
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
EC50 | effective concentration of oxidizers or fullerenols inhibited bioluminescence intensity by 50% |
F10-12 | C60,70Ox(OH)y, where x + y = 10–12, y—even |
F24-28 | C60,70Ox(OH)y, where x + y = 24-28 |
F40-42 | C60,70Ox(OH)y, where x + y = 40-42 |
FMN | flavinmononucleotide |
NADH | nicotinamide adenine dinucleotide, disodium salt, reduced |
ROS | reactive oxygen species |
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x + y | Abbreviation |
---|---|
10–12 | F10-12 |
24–28 | F24-28 |
40–42 | F40-42 |
Fullerenols | 1,4-benzoquinone | K3[Fe(CN)6] | ||
---|---|---|---|---|
Active Concentrations, g L−1 | Maximal Value of IrelOx | Active Concentrations, g L−1 | Maximal Value of IrelOx | |
F10-12 | 10–5–4·10−4 | 1.38 | - | 1.0 |
F24-28 [33] | 10–18–10−10 | 1.44 | 10−18–10−4 | 1.2 |
F40-42 [33] | 10−20–10−3 | 1.44 | - | 1.0 |
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Kovel, E.S.; Kicheeva, A.G.; Vnukova, N.G.; Churilov, G.N.; Stepin, E.A.; Kudryasheva, N.S. Toxicity and Antioxidant Activity of Fullerenol C60,70 with Low Number of Oxygen Substituents. Int. J. Mol. Sci. 2021, 22, 6382. https://doi.org/10.3390/ijms22126382
Kovel ES, Kicheeva AG, Vnukova NG, Churilov GN, Stepin EA, Kudryasheva NS. Toxicity and Antioxidant Activity of Fullerenol C60,70 with Low Number of Oxygen Substituents. International Journal of Molecular Sciences. 2021; 22(12):6382. https://doi.org/10.3390/ijms22126382
Chicago/Turabian StyleKovel, Ekaterina S., Arina G. Kicheeva, Natalia G. Vnukova, Grigory N. Churilov, Evsei A. Stepin, and Nadezhda S. Kudryasheva. 2021. "Toxicity and Antioxidant Activity of Fullerenol C60,70 with Low Number of Oxygen Substituents" International Journal of Molecular Sciences 22, no. 12: 6382. https://doi.org/10.3390/ijms22126382