Insights into the Role of the Discontinuous TM7 Helix of Human Ferroportin through the Prism of the Asp325 Residue
Abstract
:1. Introduction
2. Results
2.1. Effects of the Asp325Ala, His, and Asn Mutants on the Cell Surface Expression of Human FPN1 and Its Ability to Export Iron
2.2. 3D Structure Analysis
3. Discussion
4. Materials and Methods
4.1. Plasmid Constructs
4.2. Culture and Transfection of Human Epithelial Kidney (HEK)293T Cells
4.3. Isolation of Cell-Surface Proteins and Western Blot Analysis
4.4. Densitometry Study
4.5. 55Fe Release Measurements
4.6. 3D Structure Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
MFS | Major Facilitator Superfamily |
OF | Outward-facing |
IF | Inward-facing |
HsFPN1 | Human Ferroportin |
BbFpn | Bdellovibrio bacteriovorus Ferroportin |
TsFpn | primate Philippine Tarsier Ferroportin |
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Le Tertre, M.; Elbahnsi, A.; Ka, C.; Callebaut, I.; Le Gac, G. Insights into the Role of the Discontinuous TM7 Helix of Human Ferroportin through the Prism of the Asp325 Residue. Int. J. Mol. Sci. 2021, 22, 6412. https://doi.org/10.3390/ijms22126412
Le Tertre M, Elbahnsi A, Ka C, Callebaut I, Le Gac G. Insights into the Role of the Discontinuous TM7 Helix of Human Ferroportin through the Prism of the Asp325 Residue. International Journal of Molecular Sciences. 2021; 22(12):6412. https://doi.org/10.3390/ijms22126412
Chicago/Turabian StyleLe Tertre, Marlène, Ahmad Elbahnsi, Chandran Ka, Isabelle Callebaut, and Gérald Le Gac. 2021. "Insights into the Role of the Discontinuous TM7 Helix of Human Ferroportin through the Prism of the Asp325 Residue" International Journal of Molecular Sciences 22, no. 12: 6412. https://doi.org/10.3390/ijms22126412