Differential Regulation of Gonadotropins as Revealed by Transcriptomes of Distinct LH and FSH Cells of Fish Pituitary
Round 1
Reviewer 1 Report
In the manuscript entitled "Differential regulation of gonadotropins as revealed by transcriptomes of distinct LH and FSH cells of fish pituitary" the authors performed a transcriptome analysis of the FSH and LH gonadotroph system of Nile tilapia. Tilapia has a mono-hormonal system at the level of the pituitary, in fact LH and FSH are synthesized in different cells, unlike mammals, in which both of these hormones are secreted by the same cells. The work is very interesting and from the analysis of the findings, new insights emerge that contribute to a better understanding of the hormonal regulation of these cells, the electrical stimulatory mechanisms, and the intracellular transduction signals. Finally, the authors with the cross-study of the data present in the literature on rats identified the conserved genes, providing new information for a better understanding of the mechanisms that regulate the secretion of LH and FSH.
The work was well organized and cared for. Reading it is relatively simple and enjoyable. I would like to suggest making a list of the acronyms reported in the manuscript, to be added at the end of the work before the references.
Author Response
We would like to thank the reviewer for his comment
A detailed list of abbreviation was added at the end of the manuscript.
Reviewer 2 Report
In may opinion this is interesting and well written manuscript. Results are novel and supports main conclusion. I have only minor comments to the Authors.
- Maybe it would be worth to mention about recently discovered reproductive neuropeptide phoenixin as new player in controlling reproduction including its interaction with LH and FSH.
- Discussion seems to be a bit too long. I would suggest to make it shorter.
- A list of all abbreviations used in manuscript should be included at the end of manuscript.
- The no. of ethical approval should be provided.
- Can Authors explain why EF1α was used as reference gene during real time PCR validation?
Author Response
Please see the attachment
Author Response File: 
Author Response.docx
