MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice
Abstract
:1. Introduction
2. Results
2.1. Change of miRNA Population during Early Inflorescence Development in Rice
2.2. Identification of Differentially Expressed miRNAs and Targeted Genes Prediction
2.3. Dynamic Expression of miRNAs during Early Rice Inflorescence Development
2.4. Floral Organs Alteration in the Transgenic Lines, Overexpressing Pre-miR5506
2.5. The Abnormal Development of the Embryo Sac in the OxmiR5506 Transgenic Lines
2.6. Gene Expression Profile Change during Meiosis in the OxmiR5506 Lines
2.7. Identification of the Target Gene of miR5506
3. Discussion
3.1. A Comprehensive miRNAs Analysis Uncovers Many Uncharacterized Members That Might Affect Early Inflorescence Development in Rice
3.2. Implication of Specific miRNAs Functioning in the Development of Early Inflorescence in Rice
3.3. Rice-Specific osa-miR5506 Regulates the Development of Floral Organs and Megagametophyte
4. Materials and Methods
4.1. Plant Materials
4.2. Analysis of the Profile of miRNAs
4.3. qRT-PCR Analysis of miRNAs and Targeted Genes
4.4. Plasmid Construction and Plant Transformation
4.5. Analysis of Agronomic Traits and Cytological Observation of Embryo Sac Development
4.6. RNA-Seq Library Construction, Sequencing and Analysis
4.7. RLM-RACE
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chen, Z.; Li, Y.; Li, P.; Huang, X.; Chen, M.; Wu, J.; Wang, L.; Liu, X.; Li, Y. MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice. Int. J. Mol. Sci. 2021, 22, 6610. https://doi.org/10.3390/ijms22126610
Chen Z, Li Y, Li P, Huang X, Chen M, Wu J, Wang L, Liu X, Li Y. MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice. International Journal of Molecular Sciences. 2021; 22(12):6610. https://doi.org/10.3390/ijms22126610
Chicago/Turabian StyleChen, Zhixiong, Yajing Li, Peigang Li, Xiaojie Huang, Mingxin Chen, Jinwen Wu, Lang Wang, Xiangdong Liu, and Yajuan Li. 2021. "MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice" International Journal of Molecular Sciences 22, no. 12: 6610. https://doi.org/10.3390/ijms22126610