Macrophages Compensate for Loss of Protein Tyrosine Phosphatase N2 in Dendritic Cells to Protect from Elevated Colitis
Abstract
:1. Introduction
2. Results
2.1. Loss of PTPN2 in DCs Affects Immune Cell Infiltrations in Lamina Propria
2.2. PTPN2 Deficiency in DCs Has No Impact on Intestinal Inflammation in Acute DSS Colitis
2.3. Increased Th1 Response in PTPN2fl/flxCD11cCre Mice in Acute Colitis
2.4. DC-Specific PTPN2 Regulates Phosphorylation of STAT1 in Acute DSS Colitis
2.5. No Changes in PTPN2fl/flxCD11cCre Mice upon Induction of Chronic Inflammation
2.6. Loss of PTPN2 in DCs Has No Effect on Colitis Severity in a T Cell Transfer Colitis Model
2.7. Macrophage Depletion Results in Elevated Acute DSS Colitis in PTPN2-CD11cCre Mice
3. Discussion
4. Methods
4.1. Mice, Colitis Induction, and Macrophage Depletion
4.2. Endoscopic Assessment of Colitis Severity and Histological Score
4.3. RNA Isolation, RT-PCR, and Quantitative PCR
4.4. Protein Isolation and Western Blotting
4.5. Myeloperoxidase (MPO) Activity Assay
4.6. Immunohistochemistry
4.7. Isolation of Lamina Propria Lymphocytes
4.8. Flow Cytometry
4.9. Cell Sorting
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Hering, L.; Katkeviciute, E.; Schwarzfischer, M.; Niechcial, A.; Riggs, J.B.; Wawrzyniak, M.; Atrott, K.; van de Sande, M.; Lang, S.; Becher, B.; et al. Macrophages Compensate for Loss of Protein Tyrosine Phosphatase N2 in Dendritic Cells to Protect from Elevated Colitis. Int. J. Mol. Sci. 2021, 22, 6820. https://doi.org/10.3390/ijms22136820
Hering L, Katkeviciute E, Schwarzfischer M, Niechcial A, Riggs JB, Wawrzyniak M, Atrott K, van de Sande M, Lang S, Becher B, et al. Macrophages Compensate for Loss of Protein Tyrosine Phosphatase N2 in Dendritic Cells to Protect from Elevated Colitis. International Journal of Molecular Sciences. 2021; 22(13):6820. https://doi.org/10.3390/ijms22136820
Chicago/Turabian StyleHering, Larissa, Egle Katkeviciute, Marlene Schwarzfischer, Anna Niechcial, Julianne B. Riggs, Marcin Wawrzyniak, Kirstin Atrott, Marnix van de Sande, Silvia Lang, Burkhard Becher, and et al. 2021. "Macrophages Compensate for Loss of Protein Tyrosine Phosphatase N2 in Dendritic Cells to Protect from Elevated Colitis" International Journal of Molecular Sciences 22, no. 13: 6820. https://doi.org/10.3390/ijms22136820