Peer-Review Record
Deregulation of Secreted Frizzled-Related Protein 5 in Nonalcoholic Fatty Liver Disease Associated with Obesity
Int. J. Mol. Sci. 2021, 22(13), 6895; https://doi.org/10.3390/ijms22136895
by
Laia Bertran 1,†
, Marta Portillo-Carrasquer 1,†, Carmen Aguilar 1, José Antonio Porras 2, David Riesco 2, Salomé MartÃnez 3, Margarita Vives 4, Fà tima Sabench 4, Eva Gonzalez 5, Daniel Del Castillo 4, Cristóbal Richart 1,2 and Teresa Auguet 1,2,*
, Marta Portillo-Carrasquer 1,†, Carmen Aguilar 1, José Antonio Porras 2, David Riesco 2, Salomé MartÃnez 3, Margarita Vives 4, Fà tima Sabench 4, Eva Gonzalez 5, Daniel Del Castillo 4, Cristóbal Richart 1,2 and Teresa Auguet 1,2,*Reviewer 1:
Anonymous
Reviewer 2:
Anonymous
Int. J. Mol. Sci. 2021, 22(13), 6895; https://doi.org/10.3390/ijms22136895
Submission received: 7 June 2021
/
Revised: 23 June 2021
/
Accepted: 25 June 2021
/
Published: 27 June 2021
(This article belongs to the Special Issue Molecular Link between Steatosis and Obesity)
Round 1
Reviewer 1 Report
Why were only female subjects included in the study?Did authors look at other validated markers for SS and NASH in their samples to lend more credibility to the unusually higher SFRP5 levels seen in SS?
Table 2, 3: Include that relative expression was calculated wrt 18s RNA.
Was normality of the data checked before Spearman correlation analysis? Given the small sample size, Pearson's correlation would have been more suitable.
Could differences in the sample processing and stability explain reduced levels of SFRP5 in NASH?
Page 9 and 13: Authors highlight the differences between published studies but should discuss more on how those may explain the contradictory outcomes.
Line 361: Change to 18s RNA
Author Response
Please see the attachment
Author Response File: 
Author Response.docx
Reviewer 2 Report
The authors collected serum and liver biopsy samples from bariatric surgery patients and study the SFRP5 and JNK, and WNT5A expression. There are only some issues to be resolved.
- Table 1 shown * for significant differences between the NW group and NL group. The symbol should be in the NL, not in the NW.
- The Figure 2 protein level not consistent with the Figure 3B mRNA level of SFRP5. How do you explain that?
- Too many paragraphs to be integrated.
- The IRB numbers need to provide.
- The SS and NASH identification should be described in more detail for the definition.
Author Response
Please see the attachment
Author Response File: Author Response.docx
