Anoctamin1 Induces Hyperproliferation of HaCaT Keratinocytes and Triggers Imiquimod-Induced Psoriasis-Like Skin Injury in Mice
Abstract
:1. Introduction
2. Results
2.1. Expression of ANO1 in HaCaT Cells and Skin of Psoriatic Patient
2.2. Effects of ANO1 on HaCaT Cell Proliferation and ERK and AKT Pathways
2.3. Direct Blocking of ANO1 Channel Activity by Resveratrol, Xanthohumol, Isoxanthohumol, and Curcumin
2.4. Alleviation of Psoriasis Symptoms in Mice with Psoriatic Ears by Inhibiting ANO1 Activity
2.5. Expression of ANO1 and ANO1-Related Proteins in Psoriatic Ears of Mice
2.6. Expression of Proinflammatory Cytokines in Psoriatic Ears of Mice
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Human Tissue Samples
4.3. Cell Proliferation Assay
4.4. Knock-Down of ANO1
4.5. Halide-Sensitive YFP Imaging
4.6. Animals and IMQ-Induced Psoriasis Model Construction
4.7. Scoring Severity of Skin Inflammation
4.8. RT-PCR
4.9. Western Blot Analysis
4.10. Immunocytochemistry and Immunohistochemistry
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
ANO1 | Anoctamin1 |
BPE | Bovine pituitary extract |
BPH | Benign prostatic hyperplasia |
DAB | 3,3′-diaminobenzidine tetrahydrochloride |
DCs | Dendritic cells |
DIDS | 4,4-diisothiocyano-2,2-stilbenedisulfonic acid |
DMEM | Dulbecco’s Modified Eagle’s Medium |
FBS | Fetal bovine serum |
FRT | Fisher rat thyroid |
H&E | Hematoxylin and eosin |
HNSCC | Head and neck squamous cell carcinoma |
ICC | Immunocytochemistry |
IHC | Immunohistochemistry |
IMQ | Imiquimod |
KSFM | Keratinocyte serum free medium |
MDCs | Myeloid DCs |
NPPB | 5-nitro-2-(3-phenylpropylamino)-benzoic acid |
PASI | Psoriasis area and severity index |
PDCs | Plasmacytoid DCs |
SEM | Standard error of the mean |
si-ANO1 | siRNA targeting ANO1 |
siRNAs | Small interference RNAs |
Th | T-helper |
TMEM16A | Transmembrane protein 16A |
TNF-α | Tumor necrosis factor-α |
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Choi, M.R.; Kim, H.D.; Cho, S.; Jeon, S.H.; Kim, D.H.; Wee, J.; Yang, Y.D. Anoctamin1 Induces Hyperproliferation of HaCaT Keratinocytes and Triggers Imiquimod-Induced Psoriasis-Like Skin Injury in Mice. Int. J. Mol. Sci. 2021, 22, 7145. https://doi.org/10.3390/ijms22137145
Choi MR, Kim HD, Cho S, Jeon SH, Kim DH, Wee J, Yang YD. Anoctamin1 Induces Hyperproliferation of HaCaT Keratinocytes and Triggers Imiquimod-Induced Psoriasis-Like Skin Injury in Mice. International Journal of Molecular Sciences. 2021; 22(13):7145. https://doi.org/10.3390/ijms22137145
Chicago/Turabian StyleChoi, Mi Ran, Hae Dong Kim, Sinyoung Cho, Seong Ho Jeon, Dong Hyun Kim, Jungwon Wee, and Young Duk Yang. 2021. "Anoctamin1 Induces Hyperproliferation of HaCaT Keratinocytes and Triggers Imiquimod-Induced Psoriasis-Like Skin Injury in Mice" International Journal of Molecular Sciences 22, no. 13: 7145. https://doi.org/10.3390/ijms22137145