Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins

Jodaitis, Léni; van Oene, Thomas; Martens, Chloé

doi:10.3390/ijms22147267

Open AccessReview

Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins

by

Léni Jodaitis

^†,

Thomas van Oene

^† and

Chloé Martens

^*

Center for Structural Biology and Bioinformatics, Université Libre de Bruxelles, 1050 Brussels, Belgium

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally.

Int. J. Mol. Sci. 2021, 22(14), 7267; https://doi.org/10.3390/ijms22147267

Submission received: 31 May 2021 / Revised: 30 June 2021 / Accepted: 1 July 2021 / Published: 6 July 2021

(This article belongs to the Special Issue Membrane Proteins: Structure, Function and Motion)

Download

Browse Figures

Review Reports Versions Notes

Abstract

Membrane proteins have evolved to work optimally within the complex environment of the biological membrane. Consequently, interactions with surrounding lipids are part of their molecular mechanism. Yet, the identification of lipid–protein interactions and the assessment of their molecular role is an experimental challenge. Recently, biophysical approaches have emerged that are compatible with the study of membrane proteins in an environment closer to the biological membrane. These novel approaches revealed specific mechanisms of regulation of membrane protein function. Lipids have been shown to play a role in oligomerization, conformational transitions or allosteric coupling. In this review, we summarize the recent biophysical approaches, or combination thereof, that allow to decipher the role of lipid–protein interactions in the mechanism of membrane proteins.

Keywords: membrane protein; lipid–protein interaction; cryo-electron microscopy; hydrogen–deuterium exchange mass spectrometry; native mass spectrometry; single-molecule Förster resonance energy transfer; double electron–electron resonance; native mass spectrometry

Graphical Abstract

Share and Cite

MDPI and ACS Style

Jodaitis, L.; van Oene, T.; Martens, C. Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins. Int. J. Mol. Sci. 2021, 22, 7267. https://doi.org/10.3390/ijms22147267

AMA Style

Jodaitis L, van Oene T, Martens C. Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins. International Journal of Molecular Sciences. 2021; 22(14):7267. https://doi.org/10.3390/ijms22147267

Chicago/Turabian Style

Jodaitis, Léni, Thomas van Oene, and Chloé Martens. 2021. "Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins" International Journal of Molecular Sciences 22, no. 14: 7267. https://doi.org/10.3390/ijms22147267

APA Style

Jodaitis, L., van Oene, T., & Martens, C. (2021). Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins. International Journal of Molecular Sciences, 22(14), 7267. https://doi.org/10.3390/ijms22147267

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI