The Antiviral Molecule 5-Pyridoxolactone Identified Post BmNPV Infection of the Silkworm, Bombyx mori
Abstract
:1. Introduction
2. Results
2.1. Metabolites in the BmE Cells Infected with BmNPV
2.2. Differential Analysis of the Identified Metabolites Following BmNPV Stimulation
2.3. Pathway Analysis of the Identified Metabolites
2.4. Patterns of Metabolites in BmE Cells Infected with BmNPV at Different Times
2.5. 5-Pyridoxolactone Is an Important Antiviral Molecule in Host
2.6. 5-Pyridoxolactone May Suppress the Invasion of BmNPV
3. Discussion
4. Materials and Methods
4.1. Cells and BmNPV
4.2. Sample Collection and Metabolite Extraction
4.3. LC-MS/MS Analysis
4.4. Data Preprocessing and Analysis
4.5. Pathway Analysis
4.6. Real-Time PCR
4.7. Measurement of Viral DNA Load
4.8. Cell Toxicity and Tests
4.9. Statistics
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Hua, X.; Zhang, Q.; Xu, W.; Wang, X.; Wang, F.; Zhao, P.; Xia, Q. The Antiviral Molecule 5-Pyridoxolactone Identified Post BmNPV Infection of the Silkworm, Bombyx mori. Int. J. Mol. Sci. 2021, 22, 7423. https://doi.org/10.3390/ijms22147423
Hua X, Zhang Q, Xu W, Wang X, Wang F, Zhao P, Xia Q. The Antiviral Molecule 5-Pyridoxolactone Identified Post BmNPV Infection of the Silkworm, Bombyx mori. International Journal of Molecular Sciences. 2021; 22(14):7423. https://doi.org/10.3390/ijms22147423
Chicago/Turabian StyleHua, Xiaoting, Quan Zhang, Wei Xu, Xiaogang Wang, Fei Wang, Ping Zhao, and Qingyou Xia. 2021. "The Antiviral Molecule 5-Pyridoxolactone Identified Post BmNPV Infection of the Silkworm, Bombyx mori" International Journal of Molecular Sciences 22, no. 14: 7423. https://doi.org/10.3390/ijms22147423