Hypertrophy of Rat Skeletal Muscle Is Associated with Increased SIRT1/Akt/mTOR/S6 and Suppressed Sestrin2/SIRT3/FOXO1 Levels
Abstract
:1. Introduction
2. Results
3. Discussion
4. Methods
4.1. Animals
4.2. Synergist Muscle Ablation
4.3. NAD Measurement
4.4. Western Blots
4.5. Measurement of H2S with the Monobromobimane Method
4.6. Measurement of CBS Activity
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Time (min) | Solvent B% |
---|---|
0 | 15 |
4762 | 35 |
14,178 | 35 |
17,328 | 90 |
18,904 | 90 |
20,479 | 15 |
23,629 | 15 |
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Gombos, Z.; Koltai, E.; Torma, F.; Bakonyi, P.; Kolonics, A.; Aczel, D.; Ditroi, T.; Nagy, P.; Kawamura, T.; Radak, Z. Hypertrophy of Rat Skeletal Muscle Is Associated with Increased SIRT1/Akt/mTOR/S6 and Suppressed Sestrin2/SIRT3/FOXO1 Levels. Int. J. Mol. Sci. 2021, 22, 7588. https://doi.org/10.3390/ijms22147588
Gombos Z, Koltai E, Torma F, Bakonyi P, Kolonics A, Aczel D, Ditroi T, Nagy P, Kawamura T, Radak Z. Hypertrophy of Rat Skeletal Muscle Is Associated with Increased SIRT1/Akt/mTOR/S6 and Suppressed Sestrin2/SIRT3/FOXO1 Levels. International Journal of Molecular Sciences. 2021; 22(14):7588. https://doi.org/10.3390/ijms22147588
Chicago/Turabian StyleGombos, Zoltan, Erika Koltai, Ferenc Torma, Peter Bakonyi, Attila Kolonics, Dora Aczel, Tamas Ditroi, Peter Nagy, Takuji Kawamura, and Zsolt Radak. 2021. "Hypertrophy of Rat Skeletal Muscle Is Associated with Increased SIRT1/Akt/mTOR/S6 and Suppressed Sestrin2/SIRT3/FOXO1 Levels" International Journal of Molecular Sciences 22, no. 14: 7588. https://doi.org/10.3390/ijms22147588