Preparation of Silk Fibroin/Carboxymethyl Chitosan Hydrogel under Low Voltage as a Wound Dressing
Abstract
:1. Introduction
2. Results
2.1. Preparation of the SF/CMCS and CMCS Hydrogels
2.2. Rheological Measurements
2.3. Mechanical Properties and Infrared Spectroscopy (FTIR) of the SF/CMCS Hydrogel
2.4. Thermogravimetric Analysis (TGA)
2.5. Swelling Study and Water Vapor Transmission Rate (WVTR)
2.6. In Vitro Stability Evaluation of the Hydrogels
2.7. Antimicrobial Activity
2.8. Cytotoxicity Test
2.9. In Vivo Wound Healing Assay
3. Discussion
4. Materials and Methods
4.1. Materials and Reagents
4.2. Fabrication of SF/CMCS Hydrogel
4.2.1. Preparation of RSF Solution
4.2.2. Preparation of CMCS Solution
4.2.3. Preparation of SF/CMCS Hydrogel
4.3. Evaluation of SF Content in SF/CMCS Hydrogel
4.4. Quantifying the SF Release
4.5. Characterization of the Prepared SF/CMCS Hydrogel
4.5.1. Micromorphological Characterization
4.5.2. Rheological Characterization
4.5.3. Compressive Strength Testing
4.5.4. Fourier-Transform Infrared Spectroscopy (FTIR) Analysis
4.5.5. Thermogravimetric Analysis (TGA)
4.5.6. Swelling Studies
4.5.7. Measurement of Water Vapor Transmission Rate (WVTR)
4.5.8. Degradation Study In Vitro
4.6. Antimicrobial Activity
4.7. Cytotoxicity Assay
4.8. In Vivo Wound Healing Assay
4.8.1. Measurement of Wound Closure
4.8.2. Histological Analysis of Wounds
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Time | 10 min | 30 min | 1 h | 3 h | 6 h |
---|---|---|---|---|---|
Percentage (%) | 0.295 ± 0.023 | 0.322 ± 0.010 | 0.325 ± 0.006 | 0.352 ± 0.010 | 0.374 ± 0.022 |
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Chen, Z.; Zhang, X.; Liang, J.; Ji, Y.; Zhou, Y.; Fang, H. Preparation of Silk Fibroin/Carboxymethyl Chitosan Hydrogel under Low Voltage as a Wound Dressing. Int. J. Mol. Sci. 2021, 22, 7610. https://doi.org/10.3390/ijms22147610
Chen Z, Zhang X, Liang J, Ji Y, Zhou Y, Fang H. Preparation of Silk Fibroin/Carboxymethyl Chitosan Hydrogel under Low Voltage as a Wound Dressing. International Journal of Molecular Sciences. 2021; 22(14):7610. https://doi.org/10.3390/ijms22147610
Chicago/Turabian StyleChen, Zhenyu, Xiaoning Zhang, Jianwei Liang, Yansong Ji, Yuqing Zhou, and Hao Fang. 2021. "Preparation of Silk Fibroin/Carboxymethyl Chitosan Hydrogel under Low Voltage as a Wound Dressing" International Journal of Molecular Sciences 22, no. 14: 7610. https://doi.org/10.3390/ijms22147610