Knockout of NPFFR2 Prevents LPS-Induced Depressive-Like Responses in Mice
Abstract
:1. Introduction
2. Results
2.1. LPS Induces Depressive-Like Behaviors in WT but Not NPFFR2-KO Mice
2.2. LPS Induces Ventral Hippocampal Inflammation Responses and 5-HT1AR Changes in WT but Not NPFFR2-KO Mice
2.3. Intra-Ventral Hippocampal 5-HT1AR shRNA Pretreatment Restores LPS-Induced Depressive-Like Behavior in NPFFR2-KO Mice but Has No Effect in WT Mice
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. LPS-Induced Depressive-Like Behaviors
4.3. LPS-Induced Ventral Hippocampal Inflammation Responses and 5-HT1AR Changes
4.4. Lenti-5-HT1AR shRNA
4.5. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Target Genes | Forward Primer (5′ to 3′) | Reverse Primer (5′ to 3′) |
---|---|---|
5-HT1AR | CATGCTGGTCCTCTATGGGC | GGCTGACCATTCAGGCTCTT |
TLR4 | ATGGCATGGCTTACACCACC | GAGGCCAATTTGTCTCCACA |
TNF-α | CCGATGGGTTGTACCTTGTC | GTGGGTGAGGAGCAGTAGT |
Rpl35a | GCTGTGGTCCAAGGCCATTTT | CCGATTACTTTTCCCCAGATGAC |
Target Gene | Clone ID | Interference Sequences |
---|---|---|
5-HT1AR | TRCN0000221271 | CCCTTCCTGTTCACTCAATAT |
5-HT1AR | TRCN0000221272 | CGACCCTATAGACTACGTGAA |
5-HT1AR | TRCN0000221273 | CCTGAGTTGTTGGGTGCCATA |
5-HT1AR | TRCN0000221274 | GTACACCATCTACTCCACTTT |
5-HT1AR | TRCN0000221275 | CCCTGCTCAACCCAGTTATTT |
LacZ | TRCN0000072232 | CGTCGTATTACAACGTCGTGA |
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Yu, Z.; Lin, Y.-T.; Chen, J.-C. Knockout of NPFFR2 Prevents LPS-Induced Depressive-Like Responses in Mice. Int. J. Mol. Sci. 2021, 22, 7611. https://doi.org/10.3390/ijms22147611
Yu Z, Lin Y-T, Chen J-C. Knockout of NPFFR2 Prevents LPS-Induced Depressive-Like Responses in Mice. International Journal of Molecular Sciences. 2021; 22(14):7611. https://doi.org/10.3390/ijms22147611
Chicago/Turabian StyleYu, Zachary, Ya-Tin Lin, and Jin-Chung Chen. 2021. "Knockout of NPFFR2 Prevents LPS-Induced Depressive-Like Responses in Mice" International Journal of Molecular Sciences 22, no. 14: 7611. https://doi.org/10.3390/ijms22147611