Anti-Apoptotic and Antioxidant Activities of the Mitochondrial Estrogen Receptor Beta in N2A Neuroblastoma Cells
Abstract
:1. Introduction
2. Results
2.1. Generation of N2A Cells Stably Overexpressing a Mitochondrial-Targeted GFPERβ Protein
2.2. Effect of mtERβ on Staurosporine-Induced Apoptosis
2.3. Effect of mtERβ on H2O2-Induced Apoptosis
2.4. Effect of mtERβ on H2O2-Induced Mitochondrial Impairment in N2A Cells
2.5. Effect of Mitochondrial ERβ on ATP Content
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Antibodies
4.3. Cell Culture—Generation of N2A Cells Stably Expressing a Mitochondrial Targeted GFPERβ Fusion Protein
4.4. Western Blot Analysis
4.5. Immunofluorescence—Confocal Microscopy
4.6. Cell Viability Assay
4.7. ATP Measurements
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Tsialtas, I.; Georgantopoulos, A.; Karipidou, M.E.; Kalousi, F.D.; Karra, A.G.; Leonidas, D.D.; Psarra, A.-M.G. Anti-Apoptotic and Antioxidant Activities of the Mitochondrial Estrogen Receptor Beta in N2A Neuroblastoma Cells. Int. J. Mol. Sci. 2021, 22, 7620. https://doi.org/10.3390/ijms22147620
Tsialtas I, Georgantopoulos A, Karipidou ME, Kalousi FD, Karra AG, Leonidas DD, Psarra A-MG. Anti-Apoptotic and Antioxidant Activities of the Mitochondrial Estrogen Receptor Beta in N2A Neuroblastoma Cells. International Journal of Molecular Sciences. 2021; 22(14):7620. https://doi.org/10.3390/ijms22147620
Chicago/Turabian StyleTsialtas, Ioannis, Achilleas Georgantopoulos, Maria E. Karipidou, Foteini D. Kalousi, Aikaterini G. Karra, Demetrios D. Leonidas, and Anna-Maria G. Psarra. 2021. "Anti-Apoptotic and Antioxidant Activities of the Mitochondrial Estrogen Receptor Beta in N2A Neuroblastoma Cells" International Journal of Molecular Sciences 22, no. 14: 7620. https://doi.org/10.3390/ijms22147620