Identification of Rhythmically Expressed LncRNAs in the Zebrafish Pineal Gland and Testis
Abstract
:1. Introduction
2. Results
2.1. Rhythmically Expressed LncRNAs in the Zebrafish Pineal Gland, and Their GO, COG, and KEGG Analyses
2.2. Rhythmically Expressed lncRNAs in the Zebrafish Testis, and Their GO, COG, and KEGG Analyses
2.3. Rhythmically Expressed LncRNAs Shared between the Pineal Gland and the Testis
2.4. Conservation of Zebrafish Rhythmically Expressed LncRNAs with Mice and Humans
2.5. Conserved LncRNA-Encoded Peptides and Their Predicted 3D Structures
3. Discussion
4. Materials and Methods
4.1. Zebrafish Rhythmically Expressed RNA-Seq Datasets for the Pineal Gland and Testis
4.2. Collection of Zebrafish Pineal Gland LncRNAs
4.3. Identification of Zebrafish Testis LncRNAs
4.4. Rhythmicity Analysis
4.5. Searching for E-Box, D-Box, and RORE Regulatory Motifs in the Promoter Regions of the Rhythmically Expressed LncRNAs
4.6. GO Analysis, COG Analysis, and KEGG Pathway Analysis of Rhythmically Expressed LncRNAs
4.7. Principal Component Analysis (PCA)
4.8. Conservation Analysis with NCBI BLAST
4.9. Prediction of lncRNA-Encoded Peptides’ 3D Structure and Functions
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Mishra, S.K.; Liu, T.; Wang, H. Identification of Rhythmically Expressed LncRNAs in the Zebrafish Pineal Gland and Testis. Int. J. Mol. Sci. 2021, 22, 7810. https://doi.org/10.3390/ijms22157810
Mishra SK, Liu T, Wang H. Identification of Rhythmically Expressed LncRNAs in the Zebrafish Pineal Gland and Testis. International Journal of Molecular Sciences. 2021; 22(15):7810. https://doi.org/10.3390/ijms22157810
Chicago/Turabian StyleMishra, Shital Kumar, Taole Liu, and Han Wang. 2021. "Identification of Rhythmically Expressed LncRNAs in the Zebrafish Pineal Gland and Testis" International Journal of Molecular Sciences 22, no. 15: 7810. https://doi.org/10.3390/ijms22157810