Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (Apium graveolens L.)
Abstract
:1. Introduction
2. Results
2.1. Flower Morphology of Celery CMS Line and Maintainer Line
2.2. Microspore Development of Celery CMS Line and Maintainer Line
2.3. Mitochondrial Genome Sequence and Identification ORFs in CMS Line W99A and Its Maintainer Line W99B
2.4. Repeat Sequence Analysis of W99A and W99B Mitochondrial Genomes
2.5. Comparative Analysis of W99A and W99B Mitochondrial Genomes
2.6. SNP and InDel Detection in W99A and W99B Mitochondrial Genomes
2.7. Selection of Candidate Genes That Are Responsible for CMS Celery
2.8. Transcriptional Detection of CMS-Associated Candidate ORFs and Development a CMS Marker
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Paraffin Sectioning and Microscopy
4.3. Mitochondrial DNA Extraction and Quality Assess
4.4. Mitochondria DNA Sequence and Genome Assembly
4.5. Sequence Analysis and Genome Annotation
4.6. RT-PCR of CMS-Associated Candidate ORFs
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cheng, Q.; Wang, P.; Li, T.; Liu, J.; Zhang, Y.; Wang, Y.; Sun, L.; Shen, H. Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (Apium graveolens L.). Int. J. Mol. Sci. 2021, 22, 8584. https://doi.org/10.3390/ijms22168584
Cheng Q, Wang P, Li T, Liu J, Zhang Y, Wang Y, Sun L, Shen H. Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (Apium graveolens L.). International Journal of Molecular Sciences. 2021; 22(16):8584. https://doi.org/10.3390/ijms22168584
Chicago/Turabian StyleCheng, Qing, Peng Wang, Tiantian Li, Jinkui Liu, Yingxue Zhang, Yihao Wang, Liang Sun, and Huolin Shen. 2021. "Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (Apium graveolens L.)" International Journal of Molecular Sciences 22, no. 16: 8584. https://doi.org/10.3390/ijms22168584