Granuloma Formation in a Cyba-Deficient Model of Chronic Granulomatous Disease Is Associated with Myeloid Hyperplasia and the Exhaustion of B-Cell Lineage
Abstract
:1. Introduction
2. Results
2.1. Cyba−/− Mice Spontaneously Develop Abscesses
2.2. Cyba−/− Mice Bearing Abscesses Display Splenomegaly and Bone Marrow Myeloid Hyperplasia
2.3. Cyba−/− Mice Bearing Abscesses Undergo Drastic Changes in Haematopoiesis Homeostasis
2.4. Granuloma Induces a Sharp Decrease in Haematopoietic Progenitor Cells
2.5. Granuloma Alters B-Cell Differentiation
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Animals
4.3. Histological Analysis
4.4. Haematopoietic Lineages Analysis
4.5. Quantitative RT-PCR
4.6. Statistical Analyses and Data Report
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Prieto-Bermejo, R.; Romo-González, M.; Pérez-Fernández, A.; García-Macías, M.C.; Sánchez-Bernal, C.; García-Tuñón, I.; Sánchez-Yagüe, J.; Sánchez-Martín, M.; Hernández-Hernández, Á. Granuloma Formation in a Cyba-Deficient Model of Chronic Granulomatous Disease Is Associated with Myeloid Hyperplasia and the Exhaustion of B-Cell Lineage. Int. J. Mol. Sci. 2021, 22, 8701. https://doi.org/10.3390/ijms22168701
Prieto-Bermejo R, Romo-González M, Pérez-Fernández A, García-Macías MC, Sánchez-Bernal C, García-Tuñón I, Sánchez-Yagüe J, Sánchez-Martín M, Hernández-Hernández Á. Granuloma Formation in a Cyba-Deficient Model of Chronic Granulomatous Disease Is Associated with Myeloid Hyperplasia and the Exhaustion of B-Cell Lineage. International Journal of Molecular Sciences. 2021; 22(16):8701. https://doi.org/10.3390/ijms22168701
Chicago/Turabian StylePrieto-Bermejo, Rodrigo, Marta Romo-González, Alejandro Pérez-Fernández, María Carmen García-Macías, Carmen Sánchez-Bernal, Ignacio García-Tuñón, Jesús Sánchez-Yagüe, Manuel Sánchez-Martín, and Ángel Hernández-Hernández. 2021. "Granuloma Formation in a Cyba-Deficient Model of Chronic Granulomatous Disease Is Associated with Myeloid Hyperplasia and the Exhaustion of B-Cell Lineage" International Journal of Molecular Sciences 22, no. 16: 8701. https://doi.org/10.3390/ijms22168701