Carotenoid Cleavage Dioxygenase Genes of Chimonanthus praecox, CpCCD7 and CpCCD8, Regulate Shoot Branching in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Cloning and Phylogenetic Analysis of CpCCD7 and CpCCD8
2.2. Expression Patterns of CpCCD7 and CpCCD8
2.3. Subcellular Localization of CpCCD7 and CpCCD8
2.4. Effect of CpCCD7 and CpCCD8 Overexpression on the Branching Phenotype of Arabidopsis
2.5. CpCCD7 and CpCCD8 Genes Restore the Branching Phenotype of Arabidopsis Max Mutants
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Cloning of CpCCD7 and CpCCD8 Genes
4.3. Gene Expression Analysis
4.4. Subcellular Localization Analysis of CpCCD7 and CpCCD8 Proteins
4.5. Overexpression Plasmid Construction and Arabidopsis Transformation
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, X.; Liu, D.; Lin, J.; Zhu, T.; Liu, N.; Yang, X.; Ma, J.; Sui, S. Carotenoid Cleavage Dioxygenase Genes of Chimonanthus praecox, CpCCD7 and CpCCD8, Regulate Shoot Branching in Arabidopsis. Int. J. Mol. Sci. 2021, 22, 8750. https://doi.org/10.3390/ijms22168750
Wang X, Liu D, Lin J, Zhu T, Liu N, Yang X, Ma J, Sui S. Carotenoid Cleavage Dioxygenase Genes of Chimonanthus praecox, CpCCD7 and CpCCD8, Regulate Shoot Branching in Arabidopsis. International Journal of Molecular Sciences. 2021; 22(16):8750. https://doi.org/10.3390/ijms22168750
Chicago/Turabian StyleWang, Xia, Daofeng Liu, Jie Lin, Ting Zhu, Ning Liu, Ximeng Yang, Jing Ma, and Shunzhao Sui. 2021. "Carotenoid Cleavage Dioxygenase Genes of Chimonanthus praecox, CpCCD7 and CpCCD8, Regulate Shoot Branching in Arabidopsis" International Journal of Molecular Sciences 22, no. 16: 8750. https://doi.org/10.3390/ijms22168750