BRAF Modulates Stretch-Induced Intercellular Gap Formation through Localized Actin Reorganization
Abstract
:1. Introduction
2. Results
2.1. BRAF Knockdown Increases Cell Contractility and Actin Turnover upon Stretch in Endothelial Monolayers
2.2. ROCKII Is Required for the Phosphorylation of Cofilin upon Stretch in Endothelial Monolayers
2.3. The Presence of BRAF Accelerates the Stabilization of the Newly Formed Actin Stress Fibers after Stretch in the Stretched (Horizontal) Junctions
2.4. The Stretch-Induced Response of the Actin Cytoskeleton Results in a More Efficient Reinforcement of Cell–Cell Junctions through Excess Stress Fiber Formation in the Absence of BRAF
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cultured Cells/Cell Lines
4.3. Quantifications
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Hollósi, A.; Pászty, K.; Kellermayer, M.; Charras, G.; Varga, A. BRAF Modulates Stretch-Induced Intercellular Gap Formation through Localized Actin Reorganization. Int. J. Mol. Sci. 2021, 22, 8989. https://doi.org/10.3390/ijms22168989
Hollósi A, Pászty K, Kellermayer M, Charras G, Varga A. BRAF Modulates Stretch-Induced Intercellular Gap Formation through Localized Actin Reorganization. International Journal of Molecular Sciences. 2021; 22(16):8989. https://doi.org/10.3390/ijms22168989
Chicago/Turabian StyleHollósi, Anna, Katalin Pászty, Miklós Kellermayer, Guillaume Charras, and Andrea Varga. 2021. "BRAF Modulates Stretch-Induced Intercellular Gap Formation through Localized Actin Reorganization" International Journal of Molecular Sciences 22, no. 16: 8989. https://doi.org/10.3390/ijms22168989