Molecular Dissection of the Gene OsGA2ox8 Conferring Osmotic Stress Tolerance in Rice

Wang, Yinxiao; Du, Fengping; Wang, Juan; Li, Yingbo; Zhang, Yue; Zhao, Xiuqin; Zheng, Tianqing; Li, Zhikang; Xu, Jianlong; Wang, Wensheng; Fu, Binying

doi:10.3390/ijms22179107

Open AccessArticle

Molecular Dissection of the Gene OsGA2ox8 Conferring Osmotic Stress Tolerance in Rice

by

Yinxiao Wang

^1,2,†,

Fengping Du

^1,3,†,

Juan Wang

¹,

Yingbo Li

¹,

Yue Zhang

¹,

Xiuqin Zhao

¹,

Tianqing Zheng

¹,

Zhikang Li

^1,4,

Jianlong Xu

¹,

Wensheng Wang

^1,4,* and

Binying Fu

^1,*

¹

Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, South Zhong-Guan-Cun Street 12, Beijing 100081, China

²

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, South Zhong-Guan-Cun Street 12, Beijing 100081, China

³

College of Life Sciences, Northwest A&F University, Xianyang 712100, China

⁴

College of Agronomy, Anhui Agricultural University, Hefei 230036, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2021, 22(17), 9107; https://doi.org/10.3390/ijms22179107

Submission received: 26 July 2021 / Revised: 18 August 2021 / Accepted: 20 August 2021 / Published: 24 August 2021

(This article belongs to the Special Issue Mechanisms of Drought, Temperature and Salinity Tolerance in Plants)

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Abstract

Gibberellin 2-oxidase (GA2ox) plays an important role in the GA catabolic pathway and the molecular function of the OsGA2ox genes in plant abiotic stress tolerance remains largely unknown. In this study, we functionally characterized the rice gibberellin 2-oxidase 8 (OsGA2ox8) gene. The OsGA2ox8 protein was localized in the nucleus, cell membrane, and cytoplasm, and was induced in response to various abiotic stresses and phytohormones. The overexpression of OsGA2ox8 significantly enhanced the osmotic stress tolerance of transgenic rice plants by increasing the number of osmotic regulators and antioxidants. OsGA2ox8 was differentially expressed in the shoots and roots to cope with osmotic stress. The plants overexpressing OsGA2ox8 showed reduced lengths of shoots and roots at the seedling stage, but no difference in plant height at the heading stage was observed, which may be due to the interaction of OsGA2ox8 and OsGA20ox1, implying a complex feedback regulation between GA biosynthesis and metabolism in rice. Importantly, OsGA2ox8 was able to indirectly regulate several genes associated with the anthocyanin and flavonoid biosynthetic pathway and the jasmonic acid (JA) and abscisic acid (ABA) biosynthetic pathway, and overexpression of OsGA2ox8 activated JA signal transduction by inhibiting the expression of jasmonate ZIM domain-containing proteins. These results provide a basis for a future understanding of the networks and respective phenotypic effects associated with OsGA2ox8.

Keywords: gibberellin 2-oxidase gene; osmotic stress tolerance; jasmonate; rice

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MDPI and ACS Style

Wang, Y.; Du, F.; Wang, J.; Li, Y.; Zhang, Y.; Zhao, X.; Zheng, T.; Li, Z.; Xu, J.; Wang, W.; et al. Molecular Dissection of the Gene OsGA2ox8 Conferring Osmotic Stress Tolerance in Rice. Int. J. Mol. Sci. 2021, 22, 9107. https://doi.org/10.3390/ijms22179107

AMA Style

Wang Y, Du F, Wang J, Li Y, Zhang Y, Zhao X, Zheng T, Li Z, Xu J, Wang W, et al. Molecular Dissection of the Gene OsGA2ox8 Conferring Osmotic Stress Tolerance in Rice. International Journal of Molecular Sciences. 2021; 22(17):9107. https://doi.org/10.3390/ijms22179107

Chicago/Turabian Style

Wang, Yinxiao, Fengping Du, Juan Wang, Yingbo Li, Yue Zhang, Xiuqin Zhao, Tianqing Zheng, Zhikang Li, Jianlong Xu, Wensheng Wang, and et al. 2021. "Molecular Dissection of the Gene OsGA2ox8 Conferring Osmotic Stress Tolerance in Rice" International Journal of Molecular Sciences 22, no. 17: 9107. https://doi.org/10.3390/ijms22179107

APA Style

Wang, Y., Du, F., Wang, J., Li, Y., Zhang, Y., Zhao, X., Zheng, T., Li, Z., Xu, J., Wang, W., & Fu, B. (2021). Molecular Dissection of the Gene OsGA2ox8 Conferring Osmotic Stress Tolerance in Rice. International Journal of Molecular Sciences, 22(17), 9107. https://doi.org/10.3390/ijms22179107

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Molecular Dissection of the Gene OsGA2ox8 Conferring Osmotic Stress Tolerance in Rice

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