Keratin 8/18 Regulate the Akt Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. K18 Phosphorylation Inhibits K8/K18 Interaction with Akt Kinase
2.2. K8 and K18 as Potential Substrates of Akt
2.3. K8 G62C, R302C, and G434S Mutations, Found in Patients with Liver Diseases, Cause an Increase in the K8/K18–Akt Interaction
2.4. K8/K18–Akt Interaction Tends to Stabilize Akt
2.5. K8/K18 Expression Enhances the Phosphorylation/Activation of Akt and Its Substrates in the In Vivo Mouse System
3. Discussion
3.1. Akt-Mediated Resistance to Fas-Induced Apoptosis
3.2. A Role of K8/K18 in Tumor Progression
4. Material and Methods
4.1. List of Reagents and Antibodies
4.2. Cell Transfection and Preparation of Cell/Tissue Lysates
4.3. Co-Immunoprecipitation and Immunoblot Analysis
4.4. Protein Stability Analysis
4.5. Animals and TISSUE Collections
4.6. Site-Directed Mutagenesis
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Lim, Y.; Kim, S.; Yoon, H.-N.; Ku, N.-O. Keratin 8/18 Regulate the Akt Signaling Pathway. Int. J. Mol. Sci. 2021, 22, 9227. https://doi.org/10.3390/ijms22179227
Lim Y, Kim S, Yoon H-N, Ku N-O. Keratin 8/18 Regulate the Akt Signaling Pathway. International Journal of Molecular Sciences. 2021; 22(17):9227. https://doi.org/10.3390/ijms22179227
Chicago/Turabian StyleLim, Younglan, Sujin Kim, Han-Na Yoon, and Nam-On Ku. 2021. "Keratin 8/18 Regulate the Akt Signaling Pathway" International Journal of Molecular Sciences 22, no. 17: 9227. https://doi.org/10.3390/ijms22179227