Transcription Factor WRKY33 Mediates the Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in Arabidopsis Roots
Abstract
:1. Introduction
2. Results
2.1. WRKY33 Regulates the Pi Deficiency Responses in Arabidopsis
2.2. WRKY33 Negatively Regulates the Fe Accumulation in Roots under Pi Deficiency
2.3. WRKY33 Mediates the Plant Pi Deficiency Responses Dependent on Iron Homeostasis
2.4. WRKY33 Intersects with ALMT1 to Regulate the Plant Pi Deficiency Responses
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. RT-PCR and qRT-PCR
4.3. Pi Content Measurements
4.4. Perls and Perls/DAB Staining Assays
4.5. Root Hairs Assay
4.6. Plasmid Construction and Plant Transformation
4.7. Confocal Laser-Scanning Microscopy and Staining Procedures
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Shen, N.; Hou, S.; Tu, G.; Lan, W.; Jing, Y. Transcription Factor WRKY33 Mediates the Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in Arabidopsis Roots. Int. J. Mol. Sci. 2021, 22, 9275. https://doi.org/10.3390/ijms22179275
Shen N, Hou S, Tu G, Lan W, Jing Y. Transcription Factor WRKY33 Mediates the Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in Arabidopsis Roots. International Journal of Molecular Sciences. 2021; 22(17):9275. https://doi.org/10.3390/ijms22179275
Chicago/Turabian StyleShen, Nuo, Sifan Hou, Guoqing Tu, Wenzhi Lan, and Yanping Jing. 2021. "Transcription Factor WRKY33 Mediates the Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in Arabidopsis Roots" International Journal of Molecular Sciences 22, no. 17: 9275. https://doi.org/10.3390/ijms22179275