Identification and Characterization of MIKCc-Type MADS-Box Genes in the Flower Organs of Adonis amurensis
Abstract
:1. Introduction
2. Results
2.1. Identification and Annotation of MADS-Box Genes in A. amurensis
2.2. Expression of AaMADS Genes in the Flowers of A. amurensis
2.3. Subcellular Localization of AaMADS Proteins
2.4. Characterization of Three SOC1-Type AaMADS Genes
3. Discussion
4. Materials and Methods
4.1. Identification of MADS-Box Genes in A. amurensis
4.2. Conserved Motifs and Phylogenetic Analysis of MADS-Box Proteins
4.3. Expression-Pattern Clustering of AaMADS Genes
4.4. Quantitative Real-Time PCR (qPCR) Analysis
4.5. Gene Cloning, Vector Construction, and Plant Transformation
4.6. Subcellular Localization
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ren, L.; Sun, H.; Dai, S.; Feng, S.; Qiao, K.; Wang, J.; Gong, S.; Zhou, A. Identification and Characterization of MIKCc-Type MADS-Box Genes in the Flower Organs of Adonis amurensis. Int. J. Mol. Sci. 2021, 22, 9362. https://doi.org/10.3390/ijms22179362
Ren L, Sun H, Dai S, Feng S, Qiao K, Wang J, Gong S, Zhou A. Identification and Characterization of MIKCc-Type MADS-Box Genes in the Flower Organs of Adonis amurensis. International Journal of Molecular Sciences. 2021; 22(17):9362. https://doi.org/10.3390/ijms22179362
Chicago/Turabian StyleRen, Lulu, Hongwei Sun, Shengyue Dai, Shuang Feng, Kun Qiao, Jingang Wang, Shufang Gong, and Aimin Zhou. 2021. "Identification and Characterization of MIKCc-Type MADS-Box Genes in the Flower Organs of Adonis amurensis" International Journal of Molecular Sciences 22, no. 17: 9362. https://doi.org/10.3390/ijms22179362