Nicotine Neurotoxicity Involves Low Wnt1 Signaling in Spinal Locomotor Networks of the Postnatal Rodent Spinal Cord
Abstract
:1. Introduction
2. Results
2.1. Dose-Dependent Effect of Nicotine on Network Depolarization, Wnt1 Pathway, and Neuronal Numbers
2.2. Effect of High Nicotine Levels on Rat Spinal Neurons and Motoneurons
2.3. Upregulation of S100 and GFAP by Nicotine 10 μM
2.4. Impaired Monosynaptic Transmission and Fictive Locomotion Induced by Nic 10
2.5. Effect of Nicotine (10 μM) on Dorsal–Dorsal Root Potential
2.6. Neuronal Number, Motoneurons and Wnt1 Pathway after Excitotoxic Insult Followed by Nic 10
2.7. Poor Recovery in Reflex Responses and Fictive Locomotion
3. Discussion
3.1. An Evolving Scenario from Neuroprotection to Neurotoxicity
3.2. Nicotine, Wnt Signaling, and Neurotoxicity
3.3. Effect of High Nicotine on Kainate-Mediated Excitotoxicity
3.4. Advantages and Limitations of the Experimental Model and the Study
4. Materials and Methods
4.1. Wild-Type Rat Spinal Cord Preparation
4.2. Transgenic Mice Spinal Cord Preparation
4.3. Protocol for Drug Application and Lesioning the Spinal Cord
4.4. Electrophysiology
4.5. Immunohistochemistry
4.6. Drugs Used
4.7. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Ethical Statement
Abbreviations
BSA | Bovine serum albumin |
CO2 | Carbon dioxide |
Cre | Cre recombinase enzyme |
D-DRP | Dorsal–dorsal root potential |
DR | Dorsal root |
Elephys | Electrophysiology |
FBS | Fetal bovine serum |
iNOS | inducible nitric oxide synthase |
KA | Kainate |
L | Lumbar |
n | total number of rats/mice |
N | number of spinal slices |
nAChRs | nicotinic acetylcholine receptors |
Nic | Nicotine |
NGS | Normal goat serum |
NMDA | N-methyl-D-aspartate (NMDA) |
O2 | Oxygen |
PBS | Phosphate buffer solution |
PFA | Paraformaldehyde |
SCI | Spinal cord injury |
VR | Ventral root |
Wnt | Wingless related MMTV integration site |
5HT | 5-hydroxytryptamine |
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Kaur, J.; Mazzone, G.L.; Aquino, J.B.; Nistri, A. Nicotine Neurotoxicity Involves Low Wnt1 Signaling in Spinal Locomotor Networks of the Postnatal Rodent Spinal Cord. Int. J. Mol. Sci. 2021, 22, 9572. https://doi.org/10.3390/ijms22179572
Kaur J, Mazzone GL, Aquino JB, Nistri A. Nicotine Neurotoxicity Involves Low Wnt1 Signaling in Spinal Locomotor Networks of the Postnatal Rodent Spinal Cord. International Journal of Molecular Sciences. 2021; 22(17):9572. https://doi.org/10.3390/ijms22179572
Chicago/Turabian StyleKaur, Jaspreet, Graciela L. Mazzone, Jorge B. Aquino, and Andrea Nistri. 2021. "Nicotine Neurotoxicity Involves Low Wnt1 Signaling in Spinal Locomotor Networks of the Postnatal Rodent Spinal Cord" International Journal of Molecular Sciences 22, no. 17: 9572. https://doi.org/10.3390/ijms22179572