Netrin-1 as a Multitarget Barrier Stabilizer in the Peripheral Nerve after Injury
Abstract
:1. Introduction
2. Results
2.1. Netrin-1 Dysregulation in the Sciatic Nerve after CCI
2.2. Exogenous Recombinant Netrin-1 Treatment Rescues Neuropathic Pain via Neo1
2.3. Netrin-1 as A Multitarget Barrier Stabilizer in the Sciatic Nerve
3. Discussion
4. Materials and Methods
4.1. Chronic Constriction Injury (CCI) and Nociceptive Tests
4.2. Nociceptive Behavioral Assays
4.2.1. Thermal Nociceptive Behavioral Responses
4.2.2. Static Mechanical Hypersensitivity
4.3. Drugs and siRNA Treatment
4.4. Nerve Permeability
4.4.1. Permeability of the BNB (Perineurium)
4.4.2. Permeability of the Myelin Barrier
4.5. RNA Extraction and Quantitative PCR
4.6. Western Blotting
4.7. Immunofluorescence
4.8. Fluorescence Quantification
4.9. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Forward | Reverse |
---|---|---|
Gapdh | 5′-AGTCTACTGGCGTCTTCAC-3′ | 5′-TCATATTTCTCGTGGTTCAC-3′ |
Cldn1 | 5′-GGGACAACATCGTGACTGCT-3′ | 5′-CCACTAATGTCGCCAGACCTG-3′ |
Cldn5 | 5′-AAATTCTGGGTCTGGTGCTG-3′ | 5′-GCCGGTCAAGGTAACAAAGA-3′ |
Cldn12 | 5′-AACTGGCCAAGTGTCTGGTC-3′ | 5′-AGACCCCCTGAGCTAGCAAT-3′ |
Cldn19 | 5′-TGCTGAAGGACCCATCTG-3′ | 5′-TGTGCTTGCTGTGAGAACTG-3′ |
Ntn1 | 5′-CAGGAAGGACTATGCTGTCCA-3′ | 5′-TACGACTTGTGCCCTGCTTG-3′ |
Neo1 | 5′-TGTGATGGTGACCAAAGGCA-3′ | 5′-GGAGGCTGCCAGTTCACTATT-3′ |
Unc5b | 5′-CGACCCTAAAAGCCGCCCC-3′ | 5′-GGGATCTTGTCGGCAGAGTCC-3′ |
Unc5c | 5′-AGGCTGCTCCTGACTCAGATG-3′ | 5′-GGGTCTAGAATTGGAGAATTGG-3′ |
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Chen, J.T.-C.; Schmidt, L.; Schürger, C.; Hankir, M.K.; Krug, S.M.; Rittner, H.L. Netrin-1 as a Multitarget Barrier Stabilizer in the Peripheral Nerve after Injury. Int. J. Mol. Sci. 2021, 22, 10090. https://doi.org/10.3390/ijms221810090
Chen JT-C, Schmidt L, Schürger C, Hankir MK, Krug SM, Rittner HL. Netrin-1 as a Multitarget Barrier Stabilizer in the Peripheral Nerve after Injury. International Journal of Molecular Sciences. 2021; 22(18):10090. https://doi.org/10.3390/ijms221810090
Chicago/Turabian StyleChen, Jeremy Tsung-Chieh, Lea Schmidt, Christina Schürger, Mohammed K. Hankir, Susanne M. Krug, and Heike L. Rittner. 2021. "Netrin-1 as a Multitarget Barrier Stabilizer in the Peripheral Nerve after Injury" International Journal of Molecular Sciences 22, no. 18: 10090. https://doi.org/10.3390/ijms221810090