Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation
Abstract
:1. Introduction
2. Results
2.1. Expression Pattern of Histone Demethylase 2A (KDM2A) in White Adipose Tissues at Different Stages
2.2. Expression Pattern of KDM2A during Adipogenic Differentiation
2.3. The Subcellular Location of KDM2A In Vivo and In Vitro
2.4. Knockdown of KDM2A Inhibits the Proliferation of 3T3-L1 Preadipocytes
2.5. Knockdown of KDM2A Promotes 3T3-L1 Preadipocyte Differentiation
2.6. Deletion of KDM2A Promotes Primary Adipocytes Differentiation
2.7. Knockdown of KDM2A Promotes Adipogenesis by Affecting Expression and Nuclear Translocation of PPARγ
3. Discussion
4. Materials and Methods
4.1. Animals and Cell Line
4.2. Primary Adipocyte Isolation, Culture and Differentiation
4.3. BODIPY and Oil Red O Staining
4.4. Immunofluorescence Staining
4.5. Total RNA Extraction and Quantitative Real-Time PCR (qPCR)
4.6. Chemical Synthesis of siRNA
4.7. Cell Transfection
4.8. EdU Staining
4.9. CCK-8 Detection
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Acknowledgments
Conflicts of Interest
References
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Hua, Y.; Yue, Y.; Zhao, D.; Ma, Y.; Xiong, Y.; Xiong, X.; Li, J. Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation. Int. J. Mol. Sci. 2021, 22, 9759. https://doi.org/10.3390/ijms22189759
Hua Y, Yue Y, Zhao D, Ma Y, Xiong Y, Xiong X, Li J. Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation. International Journal of Molecular Sciences. 2021; 22(18):9759. https://doi.org/10.3390/ijms22189759
Chicago/Turabian StyleHua, Yonglin, Yongqi Yue, Dan Zhao, Yan Ma, Yan Xiong, Xianrong Xiong, and Jian Li. 2021. "Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation" International Journal of Molecular Sciences 22, no. 18: 9759. https://doi.org/10.3390/ijms22189759