Nintedanib Regulates GRK2 and CXCR2 to Reduce Neutrophil Recruitment in Endotoxin-Induced Lung Injury
Abstract
:1. Introduction
2. Results
2.1. Effects of Nintedanib on the Histopathological Features and Fibrosis of LPS-Induced ALI
2.2. Effects of Nintedanib on Neutrophil Migration in the Lung
2.3. Effects of Nintedanib on Neutrophil Accumulation in the Lung
2.4. Effect of Nintedanib on CXCR2 and GRK2 Expression Levels
2.5. Effects of Nintedanib on Human Neutrophil Migration
3. Discussion
4. Materials and Methods
4.1. Experimental Animals
4.2. Experimental Design
4.3. Histological and IHC Analyses
4.4. Lung Injury Scoring
4.5. BALF Analysis
4.6. Western Blotting
4.7. Immunofluorescence (IF) Staining
4.8. Isolation of Human Neutrophils
4.9. In Vitro Human Neutrophil Migration Assay
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Su, V.Y.-F.; Chen, W.-C.; Yu, W.-K.; Wu, H.-H.; Chen, H.; Yang, K.-Y. Nintedanib Regulates GRK2 and CXCR2 to Reduce Neutrophil Recruitment in Endotoxin-Induced Lung Injury. Int. J. Mol. Sci. 2021, 22, 9898. https://doi.org/10.3390/ijms22189898
Su VY-F, Chen W-C, Yu W-K, Wu H-H, Chen H, Yang K-Y. Nintedanib Regulates GRK2 and CXCR2 to Reduce Neutrophil Recruitment in Endotoxin-Induced Lung Injury. International Journal of Molecular Sciences. 2021; 22(18):9898. https://doi.org/10.3390/ijms22189898
Chicago/Turabian StyleSu, Vincent Yi-Fong, Wei-Chih Chen, Wen-Kuang Yu, Huai-Hsuan Wu, Hao Chen, and Kuang-Yao Yang. 2021. "Nintedanib Regulates GRK2 and CXCR2 to Reduce Neutrophil Recruitment in Endotoxin-Induced Lung Injury" International Journal of Molecular Sciences 22, no. 18: 9898. https://doi.org/10.3390/ijms22189898