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Volume 22
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10.3390/ijms221910314
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Article

Arabidopsis LSH8 Positively Regulates ABA Signaling by Changing the Expression Pattern of ABA-Responsive Proteins

by
Jinpeng Zou
1,2,3,†,
Zhifang Li
3,†,
Haohao Tang
3,†,
Li Zhang
2,3,
Jingdu Li
1,
Yuhong Li
3,
Nan Yao
3,
Yaxing Li
3,
Deguang Yang
1,* and
Zecheng Zuo
2,3,*
1
College of Agriculture, Northeast Agricultural University, Harbin 150030, China
2
Jilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun 130062, China
3
Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this paper.
Int. J. Mol. Sci. 2021, 22(19), 10314; https://doi.org/10.3390/ijms221910314
Submission received: 19 August 2021 / Revised: 19 September 2021 / Accepted: 23 September 2021 / Published: 25 September 2021
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Phytohormone ABA regulates the expression of numerous genes to significantly affect seed dormancy, seed germination and early seedling responses to biotic and abiotic stresses. However, the function of many ABA-responsive genes remains largely unknown. In order to improve the ABA-related signaling network, we conducted a large-scale ABA phenotype screening. LSH, an important transcription factor family, extensively participates in seedling development and floral organogenesis in plants, but whether its family genes are involved in the ABA signaling pathway has not been reported. Here we describe a new function of the transcription factor LSH8 in an ABA signaling pathway. In this study, we found that LSH8 was localized in the nucleus, and the expression level of LSH8 was significantly induced by exogenous ABA at the transcription level and protein level. Meanwhile, seed germination and root length measurements revealed that lsh8 mutant lines were ABA insensitive, whereas LSH8 overexpression lines showed an ABA-hypersensitive phenotype. With further TMT labeling quantitative proteomic analysis, we found that under ABA treatment, ABA-responsive proteins (ARPs) in the lsh8 mutant presented different changing patterns with those in wild-type Col4. Additionally, the number of ARPs contained in the lsh8 mutant was 397, six times the number in wild-type Col4. In addition, qPCR analysis found that under ABA treatment, LSH8 positively mediated the expression of downstream ABA-related genes of ABI3, ABI5, RD29B and RAB18. These results indicate that in Arabidopsis, LSH8 is a novel ABA regulator that could specifically change the expression pattern of APRs to positively mediate ABA responses.
Keywords: Arabidopsis; LSH8; ABA; seed germination; proteomics; ARPs Arabidopsis; LSH8; ABA; seed germination; proteomics; ARPs

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MDPI and ACS Style

Zou, J.; Li, Z.; Tang, H.; Zhang, L.; Li, J.; Li, Y.; Yao, N.; Li, Y.; Yang, D.; Zuo, Z. Arabidopsis LSH8 Positively Regulates ABA Signaling by Changing the Expression Pattern of ABA-Responsive Proteins. Int. J. Mol. Sci. 2021, 22, 10314. https://doi.org/10.3390/ijms221910314

AMA Style

Zou J, Li Z, Tang H, Zhang L, Li J, Li Y, Yao N, Li Y, Yang D, Zuo Z. Arabidopsis LSH8 Positively Regulates ABA Signaling by Changing the Expression Pattern of ABA-Responsive Proteins. International Journal of Molecular Sciences. 2021; 22(19):10314. https://doi.org/10.3390/ijms221910314

Chicago/Turabian Style

Zou, Jinpeng, Zhifang Li, Haohao Tang, Li Zhang, Jingdu Li, Yuhong Li, Nan Yao, Yaxing Li, Deguang Yang, and Zecheng Zuo. 2021. "Arabidopsis LSH8 Positively Regulates ABA Signaling by Changing the Expression Pattern of ABA-Responsive Proteins" International Journal of Molecular Sciences 22, no. 19: 10314. https://doi.org/10.3390/ijms221910314

APA Style

Zou, J., Li, Z., Tang, H., Zhang, L., Li, J., Li, Y., Yao, N., Li, Y., Yang, D., & Zuo, Z. (2021). Arabidopsis LSH8 Positively Regulates ABA Signaling by Changing the Expression Pattern of ABA-Responsive Proteins. International Journal of Molecular Sciences, 22(19), 10314. https://doi.org/10.3390/ijms221910314

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