The Impact of Multiple Functional Layers in the Structure of Magnetic Nanoparticles and Their Influence on Albumin Interaction
Abstract
:1. Introduction
2. Results and Discussion
2.1. Morphological Characterization of Fe3O4 NPs, Fe3O4@Ag NPs, and Fe3O4@Ag/TCS NPs
2.2. Evaluation of the Fe3O4 Polymorphs in the NP
2.3. Interaction between NPs and Albumin
2.3.1. Influence of Albumin Adsorption on the Surface Properties of NPs
2.3.2. Determination of the Type of Complex between NPs and Albumin
2.3.3. Determination of Interaction Affinity between NPs and Albumin
2.3.4. Determination of Type of Interaction between NPs and Albumin
3. Materials and Methods
3.1. Chemicals
3.2. Synthesis of Fe3O4, Fe3O4@Ag NPs, Fe3O4@Ag/TCS NPs
3.3. Characterization of Fe3O4, Fe3O4@Ag NPs, and Fe3O4@Ag/TCS NPs
3.3.1. Morphological Characterizations
3.3.2. Mössbauer Spectroscopy
3.4. Interaction between NPs and Albumin
3.4.1. Influence of Albumin Adsorption on Fe3O4, Fe3O4@Ag NPs, and Fe3O4@Ag/TCS
3.4.2. Spectrofluorometric Analyses of the Interaction between NPs and Albumin
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fe3O4 NPs | ||||
---|---|---|---|---|
δ (mm/s) | ΔEQ (mm/s) | BHF (T) | % Area | |
Site 1 | 0.453(7) | −0.06(2) | 50.3(1) | 42.0 |
Site 2 | −0.08(4) | 41.4(2) | 10.9 | |
Site 3 | −0.04(7) | 29.7(2) | 9.9 | |
Site 4 | 0.446(9) | −0.08(2) | 47.2(1) | 22.5 |
Site 5 | 0.13(8) | 20.6(3) | 7.0 | |
Site 6 | 0.11(7) | 8.1(2) | 7.6 | |
Fe3O4@Ag NPs | ||||
Site 1 | 0.451(5) | 0.00(1) | 52.2(1) | 59.4 |
Site 2 | −0.11(20) | 30.5(5) | 3.2 | |
Site 3 | 0.426(9) | −0.03(2) | 49.7(1) | 27.0 |
Site 4 | 0.46(36) | 20.6(1.4) | 1.1 | |
Site 5 | 0.703(34) | 0.08(8) | 42.9(3) | 5.7 |
Site 6 | −0.31(14) | 8.3(5) | 3.5 | |
Fe3O4@Ag-TCS NPs | ||||
Site 1 | 0.480(3) | 0.18(1) | 51.7(2) | 47.1 |
Site 2 | 0.17(6) | 41.4(2) | 2.6 | |
Site 3 | 0.386(4) | −0.23(1) | 51.2(1) | 36.9 |
Site 4 | 0.54(5) | 30.3(2) | 3.5 | |
Site 5 | 0.559(4) | 0.01(3) | 45.9(1) | 6.2 |
Site 6 | −0.38(5) | 8.5(2) | 3.6 |
NP | T (K) | Ksv (L mol−1) | R2 | Kq (1010 mol−1 L−1) |
---|---|---|---|---|
Fe3O4 | 298 310 330 | 1.37 × 104 1.01 × 104 1.80 × 104 | 0.99 0.99 0.99 | 13.7 10.1 18.0 |
Fe3O4@Ag | 298 310 330 | 1.14 × 104 1.23 × 104 1.59 × 104 | 0.98 0.97 0.92 | 11.4 12.3 15.9 |
Fe3O4@Ag/TCS | 298 310 330 | 2.39 × 103 2.50 × 103 6.09 × 103 | 0.98 0.92 0.95 | 2.39 2.50 6.09 |
NP | T (K) | Ka (L mol−1) | ∆H (kJ mol−1) | ∆S (J mol−1 K−1) | ∆G (kJ mol−1) | Interaction Mechanism |
---|---|---|---|---|---|---|
Fe3O4 | 298 | 7.45 × 103 | 19.63 | 140.51 | −22.09 | Hydrophobic interactions |
310 | 7.72 × 103 | −23.08 | ||||
330 | 2.08 × 104 | −27.28 | ||||
Fe3O4@Ag | 298 | 5.34 × 103 | 13.13 | 118.86 | −21.27 | Hydrophobic interactions |
310 | 7.83 × 103 | −23.11 | ||||
330 | 1.76 × 104 | −26.84 | ||||
Fe3O4@Ag/TCS | 298 | 2.62 × 103 | 25.61 | 149.78 | −19.51 | Hydrophobic interactions |
310 | 1.93 × 103 | −19.50 | ||||
330 | 7.29 × 103 | −24.41 |
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Pieretti, J.C.; Beurton, J.; Munevar, J.; Nagamine, L.C.C.M.; Le Faou, A.; Seabra, A.B.; Clarot, I.; Boudier, A. The Impact of Multiple Functional Layers in the Structure of Magnetic Nanoparticles and Their Influence on Albumin Interaction. Int. J. Mol. Sci. 2021, 22, 10477. https://doi.org/10.3390/ijms221910477
Pieretti JC, Beurton J, Munevar J, Nagamine LCCM, Le Faou A, Seabra AB, Clarot I, Boudier A. The Impact of Multiple Functional Layers in the Structure of Magnetic Nanoparticles and Their Influence on Albumin Interaction. International Journal of Molecular Sciences. 2021; 22(19):10477. https://doi.org/10.3390/ijms221910477
Chicago/Turabian StylePieretti, Joana C., Jordan Beurton, Julián Munevar, Luiz C. C. M. Nagamine, Alain Le Faou, Amedea B. Seabra, Igor Clarot, and Ariane Boudier. 2021. "The Impact of Multiple Functional Layers in the Structure of Magnetic Nanoparticles and Their Influence on Albumin Interaction" International Journal of Molecular Sciences 22, no. 19: 10477. https://doi.org/10.3390/ijms221910477