Comparative and Systematic Omics Revealed Low Cd Accumulation of Potato StMTP9 in Yeast: Suggesting a New Mechanism for Heavy Metal Detoxification
Abstract
:1. Introduction
2. Results
2.1. Chromosome Locations and Collinearity Analysis
2.2. Phylogeny Analysis and Classification of Potato MTPs
2.3. Gene Structure and Motif Analysis
2.4. The Features of StMTP Proteins
2.5. The Characteristics of Amino Acid Sequence in StMTPs Subfamily
2.6. The Cis-Acting Elements in the Promoters of StMTPs
2.7. The Response of StMTPs in Different Tissues to Heavy Metal Stress
2.8. Yeast Complementation Assay
3. Discussion
3.1. Evolutionary Analysis of Potato MTP Family
3.2. Conservative StMTP9 and Specific StMTP8
3.3. Co-Expression Network Analysis under Cd and Zn Stress
3.4. Special Cadmium Tolerance of StMTP9 in Yeast Mutants
3.5. Conclusions
4. Materials and Methods
4.1. Identification of MTP Gene Family Members and Collinearity Analysis
4.2. Phylogenetic Analysis of the MTP Gene Family
4.3. The Gene Structures and Motif Analysis in Potato and Arabidopsis
4.4. The Characterization of Predicted StMTP Proteins
4.5. Comparison of Potato and Arabidopsis Protein Sequences
4.6. Analysis of Cis-Acting Elements of StMTPs
4.7. Obtaining and Processing Methods of Test Materialss
4.8. Yeast Complementation Assay
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Transcript ID | MW (KDa) | PI | Instability Index | GRAVY | Subcellular Localization | TMD Number |
---|---|---|---|---|---|---|---|
StMTP1 | PGSC0003DMT400081988 | 45.43 | 5.07 | 43.26 | 0.0300 | Vacuole | 5 |
StMTP2 | PGSC0003DMT400017877 | 100.11 | 6.97 | 42.20 | −0.0400 | Vacuole | 16 |
StMTP3 | PGSC0003DMT400002845 | 44.83 | 5.01 | 48.32 | 0.1200 | Vacuole | 4 |
StMTP4 | PGSC0003DMT400038744 | 28.77 | 7.17 | 46.81 | −0.2100 | Vacuole | 2 |
StMTP5 | PGSC0003DMT400060459 | 38.96 | 5.22 | 44.16 | 0.2400 | Vacuole | 4 |
StMTP6 | PGSC0003DMT400077991 | 42.50 | 5.85 | 28.80 | 0.1400 | Vacuole | 6 |
StMTP7 | PGSC0003DMT400078896 | 45.97 | 6.05 | 28.74 | 0.0050 | Vacuole | 6 |
StMTP8 | PGSC0003DMT400078996 | 39.47 | 5.97 | 34.53 | 0.1900 | Vacuole | 5 |
StMTP9 | PGSC0003DMT400029243 | 51.40 | 6.63 | 46.16 | 0.0300 | Cell membrane; Vacuole | 7 |
StMTP10 | PGSC0003DMT400024996 | 40.00 | 8.96 | 42.90 | −0.0200 | Cell membrane; Vacuole | 4 |
StMTP11 | PGSC0003DMT400010945 | 46.38 | 5.35 | 46.56 | 0.0300 | Vacuole | 4 |
StMTPs | Similar Functions to Model Plants | Structure Domain (HXXXD or DXXXXD) | Zinc-Rich Area | ZT_Dimer | Signal Peptide | Improve Yeast Cadmium Tolerance |
---|---|---|---|---|---|---|
StMTP2 | Forms dimers with StMTP4 to transport Zn2+ | HXXXD not exists on TMD2 or/and TMD5 | Yes | No | No | - |
StMTP4 | Forms dimers with StMTP2 to transport Zn2+ | HXXXD not exists on TMD2 or/and TMD5 | No | No | No | - |
StMTP5 | Transport the heavy metals | DXXXD exists on TMD2 and TMD5 | No | Yes | No | - |
StMTP8 | Transport Zn2+ | HXXXD not exists on TMD2 or/and TMD5 | Yes | No | Yes | High tolerance; high accumulation |
StMTP9 | Transport Mn2+ | DXXXD exists on TMD2 | No | Yes | No | High tolerance; low accumulation |
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Li, D.; He, G.; Tian, W.; Saleem, M.; Huang, Y.; Meng, L.; Wu, D.; He, T. Comparative and Systematic Omics Revealed Low Cd Accumulation of Potato StMTP9 in Yeast: Suggesting a New Mechanism for Heavy Metal Detoxification. Int. J. Mol. Sci. 2021, 22, 10478. https://doi.org/10.3390/ijms221910478
Li D, He G, Tian W, Saleem M, Huang Y, Meng L, Wu D, He T. Comparative and Systematic Omics Revealed Low Cd Accumulation of Potato StMTP9 in Yeast: Suggesting a New Mechanism for Heavy Metal Detoxification. International Journal of Molecular Sciences. 2021; 22(19):10478. https://doi.org/10.3390/ijms221910478
Chicago/Turabian StyleLi, Dandan, Guandi He, Weijun Tian, Muhammad Saleem, Yun Huang, Lulu Meng, Danxia Wu, and Tengbing He. 2021. "Comparative and Systematic Omics Revealed Low Cd Accumulation of Potato StMTP9 in Yeast: Suggesting a New Mechanism for Heavy Metal Detoxification" International Journal of Molecular Sciences 22, no. 19: 10478. https://doi.org/10.3390/ijms221910478