Establishment of Bovine-Induced Pluripotent Stem Cells
Abstract
:1. Introduction
2. Results
2.1. Establishment of Bovine iPSCs
2.2. Characterization of Primed-like Bovine iPSCs
2.3. Development and Characterization of Naïve-Like Bovine iPSCs
2.4. Global Transcriptome Analysis of Bovine iPSCs
2.5. In Vivo Chimerism Capacity of Bovine iPSCs in Mouse Embryos
2.6. Efficiency of biPSCs as Donors for Somatic Cell Nuclear Transfer (SCNT)
3. Discussion
4. Materials and Methods
4.1. Chemicals, DNA Constructs, and Primary Bovine Cells
4.2. Reprogramming of bMSCs
4.3. Quantitative Reverse Transcription-PCR (qRT-PCR) Analysis
4.4. Embryoid Body (EB) Differentiation
4.5. Immunostaining
4.6. RNA-seq Data Analysis
4.7. Karyotyping
4.8. Bisulfite Sequencing
4.9. Aggregation to Generate Chimeric Embryos
4.10. Nuclear Transfer Using biPSCs as Nuclear Donors
4.11. Statistial Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Donor Cells | No. of Oocytes | No. of Fused (mean ± sd%) | No. of Reconstructed Embryos | No. of Cleaved Embryos (mean ± sd%) | No. of Blastocysts (mean ± sd%) * |
---|---|---|---|---|---|
DsRed-biPSC | 194 | 170 (88.4 ± 5.4) a | 123 | 89 (70.5 ± 17.0) a | 22 (27.0 ± 9.0) a |
GFP-Dox-biPSC | 206 | 183 (88.9 ± 1.9) a | 131 | 98 (74.9 ± 10.8) a | 24 (25.2 ± 15.2) a |
bAF | 188 | 172 (91.5 ± 0.5) a | 125 | 111 (88.6 ± 2.0) a | 45 (39.3 ± 10.7) a |
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Su, Y.; Wang, L.; Fan, Z.; Liu, Y.; Zhu, J.; Kaback, D.; Oudiz, J.; Patrick, T.; Yee, S.P.; Tian, X.; et al. Establishment of Bovine-Induced Pluripotent Stem Cells. Int. J. Mol. Sci. 2021, 22, 10489. https://doi.org/10.3390/ijms221910489
Su Y, Wang L, Fan Z, Liu Y, Zhu J, Kaback D, Oudiz J, Patrick T, Yee SP, Tian X, et al. Establishment of Bovine-Induced Pluripotent Stem Cells. International Journal of Molecular Sciences. 2021; 22(19):10489. https://doi.org/10.3390/ijms221910489
Chicago/Turabian StyleSu, Yue, Ling Wang, Zhiqiang Fan, Ying Liu, Jiaqi Zhu, Deborah Kaback, Julia Oudiz, Tayler Patrick, Siu Pok Yee, Xiuchun (Cindy) Tian, and et al. 2021. "Establishment of Bovine-Induced Pluripotent Stem Cells" International Journal of Molecular Sciences 22, no. 19: 10489. https://doi.org/10.3390/ijms221910489