Incomptine A Induces Apoptosis, ROS Production and a Differential Protein Expression on Non-Hodgkin’s Lymphoma Cells †
Abstract
:1. Introduction
2. Results and Discussion
3. Conclusions
4. Materials and Methods
4.1. Incomptine A (IA) Isolation
4.2. Chemicals
4.3. Cell Cultures and Reagents
4.3.1. Cytotoxic Activity
4.3.2. Annexin V/DAPI Apoptosis Assay
4.3.3. Reactive Oxygen Species Assay
4.3.4. Cell Lysis and iTRAQ-Based Proteomic Analysis
4.3.5. Nano LC-MS/MS Analyses
4.3.6. Protein Identification
4.3.7. Differential Protein Analysis
4.3.8. Statistical Analysis
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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NHL Cell Line | Incomptine A (IA) CC50 (mM) a | Methotrexate CC50 (mM) a |
---|---|---|
U-937 | 0.12 ± 0.02 | 0.5 ± 0.004 |
Farage | 2.3 ± 0.55 | 1.5 ± 0.01 |
SU-DHL-2 | 3.2 ± 0.04 | 1.3 ± 0.01 |
REC-1 | 3.5 ± 0.01 | 8.1 ± 0.05 |
Protein ID | Protein Name | Gene Name | MTX (FC) | IA (FC) |
---|---|---|---|---|
Q07812 | Apoptosis regulator BAX | BAX | 1.12 | 1.75 |
Q53EL6 | Programmed cell death protein 4 | PDCD4 | 1.98 | 2.80 |
Q92597 | NDRG1 protein | NDRG1 | 1.53 | 1.64 |
Q16548 | Bcl-2-related protein A1 | BCL2A1 | 0.77 | 0.53 |
Q9NYF8 | Bcl-2-associated transcription factor 1 | BCLAF1 | 0.56 | 0.26 |
P00374 | Dihydrofolate reductase | DHFR | 1.42 | 1.77 |
P11586 | Tetrahydrofolate synthase | MTHFD1 | 1.29 | 2.1 |
P60891 | Ribose phosphate pyrophosphokinase 1 | PRPS1 | 1.65 | 2.24 |
P10620 | Microsomal glutathione S-transferase 1 | MGST1 | 0.76 | 0.59 |
Q9Y2Q3 | Glutathione S-transferase kappa 1 | GSTK1 | 0.65 | 0.49 |
Q06830 | Peroxiredoxin-1 | PRDX1 | 0.93 | 2.74 |
P32119 | Peroxiredoxin-2 | PRDX2 | 1.21 | 1.95 |
P30041 | Peroxiredoxin-6 | PRDX6 | 1.27 | 2.29 |
O14920 | Inhibitor of nuclear factor kappa-B kinase subunit beta | IKBKB | 1.23 | 1.73 |
P19838 | Nuclear factor NF-kappa-B p105 subunit | NFKB1 | 1.11 | 1.68 |
Q04206 | Nuclear factor NF-kappa-B p65 subunit | RELA | 1.79 | 1.62 |
Q04864 | c-Rel | REL | 1.45 | 1.9 |
P42224 | Signal transducer and activator of transcription 1-alpha/beta | STAT1 | 1.50 | 1.97 |
P30281 | G1/S-specific cyclin-D3 | CCND3 | 1.21 | 0.57 |
P43246 | DNA mismatch repair protein Msh2 | MSH2 | 1.39 | 1.95 |
O95352 | Autophagy protein 7 | ATG7 | 1.40 | 2.73 |
Q9NT62 | Autophagy protein 3 | ATG3 | 1.31 | 2.21 |
P11279 | Lysosomal associated membrane protein 1 | LAMP1 | 0.91 | 1.60 |
P13473 | Lysosomal associated membrane protein 2 | LAMP2 | 1.47 | 1.86 |
P00338 | L-lactate dehydrogenase A chain | LDHA | 0.97 | 0.54 |
P07195 | L-lactate dehydrogenase B chain | LDHB | 0.78 | 0.51 |
P04075 | Fructose-bisphosphate aldolase A | ALDOA | 1.06 | 0.34 |
P18669 | Phosphoglycerate mutase 1 | PGAM1 | 1.25 | 0.62 |
O43707 | Alpha actinin 4 | ACTN4 | 1.27 | 0.51 |
Q6IRU2 | Tropomyosin alpha-4 chain | TPM4 | 0.96 | 0.43 |
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Pina-Jiménez, E.; Calzada, F.; Bautista, E.; Ordoñez-Razo, R.M.; Velázquez, C.; Barbosa, E.; García-Hernández, N. Incomptine A Induces Apoptosis, ROS Production and a Differential Protein Expression on Non-Hodgkin’s Lymphoma Cells. Int. J. Mol. Sci. 2021, 22, 10516. https://doi.org/10.3390/ijms221910516
Pina-Jiménez E, Calzada F, Bautista E, Ordoñez-Razo RM, Velázquez C, Barbosa E, García-Hernández N. Incomptine A Induces Apoptosis, ROS Production and a Differential Protein Expression on Non-Hodgkin’s Lymphoma Cells. International Journal of Molecular Sciences. 2021; 22(19):10516. https://doi.org/10.3390/ijms221910516
Chicago/Turabian StylePina-Jiménez, Emmanuel, Fernando Calzada, Elihú Bautista, Rosa María Ordoñez-Razo, Claudia Velázquez, Elizabeth Barbosa, and Normand García-Hernández. 2021. "Incomptine A Induces Apoptosis, ROS Production and a Differential Protein Expression on Non-Hodgkin’s Lymphoma Cells" International Journal of Molecular Sciences 22, no. 19: 10516. https://doi.org/10.3390/ijms221910516