mRNA and miRNA Expression Analyses of the MYC/E2F/miR-17-92 Network in the Most Common Pediatric Brain Tumors
Abstract
:1. Introduction
2. Results
2.1. MYC and E2F Gene Expression Is Connected with Tumor Type and Grade
2.2. miRNA Expression Depends on Tumor’s Histopathology and WHO Grade
2.3. Relationship between Gene Expression of Genes from MYC and E2F Families and miRNAs
3. Discussion
4. Materials and Methods
4.1. Patients and Tissue Samples
4.2. RNA Isolation and Reverse Transcription
4.3. Reverse Transcription and Quantification of Gene Expression by qRT-PCR
4.4. Reverse Transcription and Detection of miRNA Expression by qRT-PCR
4.5. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
EP | Ependymoma |
MB | Medulloblastoma |
PA | Pilocytic astrocytoma |
References
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Gene | MB vs. PA | MB vs. EP | EP vs. PA |
---|---|---|---|
MYCC | 0.788019 | 0.471420 | 0.164877 |
MYCN | 0.012457 | 0.375058 | 0.535525 |
MYCL | 0.011674 | 0.000109 | 0.044045 |
E2F1 | 0.000000 | 0.013521 | 0.014328 |
E2F2 | 0.000000 | 0.000023 | 1.000000 |
E2F3 | 0.132115 | 0.010907 | 1.000000 |
miRNA | MB vs. PA | MB vs. EP | EP vs. PA | |
---|---|---|---|---|
17-92 | miR-17-5p | 0.0000,66 | 1.0000,00 | 0.0018,81 |
miR-18a | 0.0112,56 | 0.2688,29 | 0.6894,59 | |
miR-19a | 0.0002,08 | 0.2688,29 | 0.6894,59 | |
miR-20a | 0.0000,10 | 0.7671,39 | 0.0013,14 | |
miR-19b | 0.0001,28 | 0.0765,68 | 0.0343,83 | |
miR-92 | 0.0030,20 | 1.0000,00 | 0.0288,40 | |
106b-25 | miR-106b | 0.0001,07 | 0.1327,58 | 0.0001,70 |
miR-93 | 0.0001,07 | 0.0076,12 | 0.0003,19 | |
miR-25 | 0.0001,07 | 0.0526,61 | 0.0002,33 | |
106a-363 | miR-106a | 0.0001,07 | 0.0372,52 | 0.0042,90 |
miR-18b | 0.0083,77 | 0.1771,93 | 0.8113,97 | |
miR-19b-2 A | 0.0001,28 | 0.0765,68 | 0.0343,83 | |
miR-20b | 0.0837,45 | 0.3829,15 | 0.6841,70 | |
miR-92-2 B | 0.0030,20 | 1.0000,00 | 0.0288,40 | |
miR-363 | 0.0000,00 | 0.0000,00 | 1.0000,00 |
Gene–Gene | MB | EP | PA |
---|---|---|---|
MYCC-E2F1 | −0.21 | 0.55 | 0.75 |
MYCC-E2F2 | −0.13 | 0.30 | 0.61 |
MYCC-E2F3 | 0.19 | 0.54 | 0.72 |
MYCN-E2F1 | 0.40 | 0.66 | 0.81 |
MYCN-E2F2 | 0.51 | 0.60 | 0.79 |
MYCN-E2F3 | −0.21 | 0.55 | 0.79 |
MYCL-E2F1 | 0.21 | 0.25 | 0.37 |
MYCL-E2F2 | 0.38 | 0.29 | 0.55 |
MYCL-E2F3 | −0.22 | 0.62 | 0.60 |
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Gruszka, R.; Zakrzewski, K.; Liberski, P.P.; Zakrzewska, M. mRNA and miRNA Expression Analyses of the MYC/E2F/miR-17-92 Network in the Most Common Pediatric Brain Tumors. Int. J. Mol. Sci. 2021, 22, 543. https://doi.org/10.3390/ijms22020543
Gruszka R, Zakrzewski K, Liberski PP, Zakrzewska M. mRNA and miRNA Expression Analyses of the MYC/E2F/miR-17-92 Network in the Most Common Pediatric Brain Tumors. International Journal of Molecular Sciences. 2021; 22(2):543. https://doi.org/10.3390/ijms22020543
Chicago/Turabian StyleGruszka, Renata, Krzysztof Zakrzewski, Paweł Piotr Liberski, and Magdalena Zakrzewska. 2021. "mRNA and miRNA Expression Analyses of the MYC/E2F/miR-17-92 Network in the Most Common Pediatric Brain Tumors" International Journal of Molecular Sciences 22, no. 2: 543. https://doi.org/10.3390/ijms22020543