Biochemical and Behavioral Consequences of Ethanol Intake in a Mouse Model of Metabolic Syndrome
Abstract
:1. Introduction
2. Results
2.1. Effects of Ethanol Intake with Regard to Weight, Glucose Overdose, Insulin, and Basal Glycemia
2.2. Cognitive Effects of Ethanol Consumption
2.3. Effects of Ethanol Intake on GR and GPx Activity
2.4. Effects of Ethanol Consumption on GSH, GSSG, GSH/GSSG Ratio, and L-Cysteine
2.5. Effects of Ethanol Administration on MDA Levels
3. Discussion
3.1. MS Mice Model and Chronic Ethanol Consumption
3.2. Chronic Ethanol Consumption and MS Modulate Oxidative Status in the Brain of Mice
3.3. Behavioral Effects of Chronic Ethanol Consumption in MS Mice
4. Materials and Methods
4.1. Animals
4.2. Drugs and Chemicals
4.3. Experimental Procedure
4.3.1. Glycemia, Insulinemia, and Glucose Overload Tests
4.3.2. Memory and Learning Test
4.3.3. Antioxidant Defenses
GSH, GSSG, and L-Cysteine
Glutathione Peroxidase Activity (GPx)
Glutathione Reductase Activity (GR)
4.3.4. Lipid Peroxidation
4.3.5. Protein Content
4.3.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
cAMP | Cyclic adenosine monophosphate |
DI | Discrimination index |
DTNB | 5,5′-dithiobis(2-nitrobenzoic acid) |
EDTA | Ethylenediaminetetraacetic acid |
GABA | Γ-aminobutyric acid |
GPx | Glutathione peroxidase activity |
GR | Glutathione reductase activity |
GSH | Glutathione |
GSSG | Glutathione disulfide |
HPLC | High-performance liquid chromatography |
L-Cys | L-cysteine |
MDA | Malondialdehyde |
MS | Metabolic syndrome |
NADPH | Nicotinamide adenine dinucleotide phosphate |
T2D | Type 2 diabetes |
WHO | World Health Organization |
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Baliño, P.; Romero-Cano, R.; Muriach, M. Biochemical and Behavioral Consequences of Ethanol Intake in a Mouse Model of Metabolic Syndrome. Int. J. Mol. Sci. 2021, 22, 807. https://doi.org/10.3390/ijms22020807
Baliño P, Romero-Cano R, Muriach M. Biochemical and Behavioral Consequences of Ethanol Intake in a Mouse Model of Metabolic Syndrome. International Journal of Molecular Sciences. 2021; 22(2):807. https://doi.org/10.3390/ijms22020807
Chicago/Turabian StyleBaliño, Pablo, Ricard Romero-Cano, and María Muriach. 2021. "Biochemical and Behavioral Consequences of Ethanol Intake in a Mouse Model of Metabolic Syndrome" International Journal of Molecular Sciences 22, no. 2: 807. https://doi.org/10.3390/ijms22020807