Multiplexed MRM-Based Proteomics Identified Multiple Biomarkers of Disease Severity in Human Heart Failure
Abstract
:1. Introduction
2. Results
2.1. Characteristics of Study Participants
2.2. Analysis of Plasma Biomarkers in Relation to HF Severity
2.3. Diagnostic Performance of the Plasma Biomarkers
2.4. Interaction Network Analysis
3. Discussion
4. Materials and Methods
4.1. Study Population
4.2. Sample Preparation
4.3. Liquid Chromatography Mass Spectrometry (LC-MS) Analysis
4.4. Computational Analysis
4.5. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Controls | Low Severity HF | Medium Severity HF | High Severity HF | |||
---|---|---|---|---|---|---|
Protein | VO2/Kg > 16 mL/min/Kg (n = 9) | 12 < VO2/Kg < 16 mL/min/Kg (n = 7) | VO2/Kg < 12 mL/min/Kg (n = 6) | p-Value | Adjusted p-Value | |
A1AC | 1.332 ± 0.323 | 1.3466 ± 0.282 | 1.742 ± 0.471 | 2.030 ± 0.270 | 0.002 | 0.001 |
A2M | 0.946 ± 0.163 | 1.055 ± 0.356 | 1.028 ± 0.169 | 1.398 ± 0.475 | ns | - |
B2M | 0.873 ± 0.319 | 1.073 ± 0.429 | 1.5058 ± 0.663 | 2.665 ± 1.240 | <0.001 | 0.001 |
C9 | 0.768 ± 0.192 | 0.7955 ± 0.282 | 0.9268 ± 0.314 | 1.171 ± 0.0854 | 0.020 | 0.014 |
GSN | 1.034 ± 0.174 | 0.760 ± 0.185 | 0.822 ± 0.200 | 0.729 ± 0.216 | 0.020 | 0.043 |
IGFBP3 | 0.929 ± 0.0930 | 0.774 ± 0.124 | 0.849 ± 0.340 | 0.589 ± 0.335 | ns | - |
IGFALS | 1.101 ± 0.251 | 0.851 ± 0.250 | 0.963 ± 0.437 | 0.696 ± 0.390 | ns | - |
NRP2 | 0.448 ± 0.0855 | 0.520 ± 0.076 | 0.667 ± 0.250 | 0.789 ± 0.112 | 0.001 | < 0.001 |
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Brioschi, M.; Gianazza, E.; Agostoni, P.; Zoanni, B.; Mallia, A.; Banfi, C. Multiplexed MRM-Based Proteomics Identified Multiple Biomarkers of Disease Severity in Human Heart Failure. Int. J. Mol. Sci. 2021, 22, 838. https://doi.org/10.3390/ijms22020838
Brioschi M, Gianazza E, Agostoni P, Zoanni B, Mallia A, Banfi C. Multiplexed MRM-Based Proteomics Identified Multiple Biomarkers of Disease Severity in Human Heart Failure. International Journal of Molecular Sciences. 2021; 22(2):838. https://doi.org/10.3390/ijms22020838
Chicago/Turabian StyleBrioschi, Maura, Erica Gianazza, Piergiuseppe Agostoni, Beatrice Zoanni, Alice Mallia, and Cristina Banfi. 2021. "Multiplexed MRM-Based Proteomics Identified Multiple Biomarkers of Disease Severity in Human Heart Failure" International Journal of Molecular Sciences 22, no. 2: 838. https://doi.org/10.3390/ijms22020838