Purification and Biochemical Characterization of Sucrose synthase from the Stem of Nettle (Urtica dioica L.)

Mareri, Lavinia; Guerriero, Gea; Hausman, Jean-Francois; Cai, Giampiero

doi:10.3390/ijms22020851

Open AccessArticle

Purification and Biochemical Characterization of Sucrose synthase from the Stem of Nettle (Urtica dioica L.)

¹

Dipartimento Scienze della Vita, Università di Siena, via Mattioli 4, 53100 Siena, Italy

²

Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 5 rue Bommel, Z.A.E. Robert Steichen, L-4940 Hautcharage, Luxembourg

^*

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2021, 22(2), 851; https://doi.org/10.3390/ijms22020851

Submission received: 27 November 2020 / Revised: 11 January 2021 / Accepted: 12 January 2021 / Published: 16 January 2021

(This article belongs to the Section Molecular Plant Sciences)

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Abstract

Sucrose synthase is a key enzyme in sucrose metabolism as it saves an important part of sucrose energy in the uridine-5′-diphosphate glucose (UDP-glucose) molecule. As such it is also involved in the synthesis of fundamental molecules such as callose and cellulose, the latter being present in all cell walls of plant cells and therefore also in the gelatinous cell walls of sclerenchyma cells such as bast fibers. Given the importance of these cells in plants of economic interest such as hemp, flax and nettle, in this work we have studied the occurrence of Sucrose synthase in nettle stems by analyzing its distribution between the cytosol, membranes and cell wall. We have therefore developed a purification protocol that can allow the analysis of various characteristics of the enzyme. In nettle, Sucrose synthase is encoded by different genes and each form of the enzyme could be subjected to different post-translational modifications. Therefore, by two-dimensional electrophoresis analysis, we have also traced the phosphorylation profile of Sucrose synthase isoforms in the various cell compartments. This information paves the way for further investigation of Sucrose synthase in plants such as nettle, which is both economically important, but also difficult to study.

Keywords: Urtica dioica; Sucrose synthase; bast fibers; protein purification; phosphorylation

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MDPI and ACS Style

Mareri, L.; Guerriero, G.; Hausman, J.-F.; Cai, G. Purification and Biochemical Characterization of Sucrose synthase from the Stem of Nettle (Urtica dioica L.). Int. J. Mol. Sci. 2021, 22, 851. https://doi.org/10.3390/ijms22020851

AMA Style

Mareri L, Guerriero G, Hausman J-F, Cai G. Purification and Biochemical Characterization of Sucrose synthase from the Stem of Nettle (Urtica dioica L.). International Journal of Molecular Sciences. 2021; 22(2):851. https://doi.org/10.3390/ijms22020851

Chicago/Turabian Style

Mareri, Lavinia, Gea Guerriero, Jean-Francois Hausman, and Giampiero Cai. 2021. "Purification and Biochemical Characterization of Sucrose synthase from the Stem of Nettle (Urtica dioica L.)" International Journal of Molecular Sciences 22, no. 2: 851. https://doi.org/10.3390/ijms22020851

APA Style

Mareri, L., Guerriero, G., Hausman, J.-F., & Cai, G. (2021). Purification and Biochemical Characterization of Sucrose synthase from the Stem of Nettle (Urtica dioica L.). International Journal of Molecular Sciences, 22(2), 851. https://doi.org/10.3390/ijms22020851

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Purification and Biochemical Characterization of Sucrose synthase from the Stem of Nettle (Urtica dioica L.)

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