The Drosophila miR-959–962 Cluster Members Repress Toll Signaling to Regulate Antibacterial Defense during Bacterial Infection
Abstract
:1. Introduction
2. Results
2.1. The miR-959–962 Cluster Could Negatively Regulate Drosophila Toll-Related Immune Response
2.2. Each Member from miR-959–962 Cluster Plays a Negative Regulatory Role in Drosophila Toll Pathway
2.3. The Immune-Related Genes Are Potentially Targeted by miRNA Members from miR-959–962 Cluster In Vitro
2.4. The miR-959–962 Member Simultaneously or Serperately Target Key Components of Toll Pathway (Tube, dl, and Toll) In Vivo
2.5. Dynamic Expression Patterns of miR-959–962 Cluster Members in Wild-Type Flies after M. luteus or PBS Infection
3. Discussion
4. Materials and Methods
4.1. Drosophila Stocks and Husbandry
4.2. Adult Immune Challenge
4.3. Quantitative RT–PCR Analysis
4.4. miRNA Targets Prediction
4.5. Recombinant Plasmids Generation
4.6. Cell Transfection and Luciferase Assays
4.7. Data Processing and Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
miRNA | microRNA |
Drs | Drosomycin |
dl | dorsal |
3′ UTR | 3′ untranslated region |
imd | immune deficiency |
AMP | antimicrobial peptide |
M. luteus | Micrococcus luteus |
E. faecalis | Enterococcus faecalis |
Drs-GFP | Drosomycin–green fluorescent protein |
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Li, R.; Yao, X.; Zhou, H.; Jin, P.; Ma, F. The Drosophila miR-959–962 Cluster Members Repress Toll Signaling to Regulate Antibacterial Defense during Bacterial Infection. Int. J. Mol. Sci. 2021, 22, 886. https://doi.org/10.3390/ijms22020886
Li R, Yao X, Zhou H, Jin P, Ma F. The Drosophila miR-959–962 Cluster Members Repress Toll Signaling to Regulate Antibacterial Defense during Bacterial Infection. International Journal of Molecular Sciences. 2021; 22(2):886. https://doi.org/10.3390/ijms22020886
Chicago/Turabian StyleLi, Ruimin, Xiaolong Yao, Hongjian Zhou, Ping Jin, and Fei Ma. 2021. "The Drosophila miR-959–962 Cluster Members Repress Toll Signaling to Regulate Antibacterial Defense during Bacterial Infection" International Journal of Molecular Sciences 22, no. 2: 886. https://doi.org/10.3390/ijms22020886