Mechanical Stress Induces Ca2+-Dependent Signal Transduction in Erythroblasts and Modulates Erythropoiesis
Abstract
:1. Introduction
2. Results
2.1. Extended PIEZO1 Activation by Yoda1 and Mechanical Stress Negatively Affects Erythroblasts Proliferation
2.2. Mechanical Stimulation and Yoda1 Incubation Induce Ca2+-Dependent Signaling Pathways
2.3. Intracellular Ca2+ Chelation Prevents Shear-Induced Signaling Pathways
3. Discussion
3.1. How to Quantify Mechanical Stress?
3.2. Control of Intracellular Ca2+ Levels
3.3. Signal Transduction Downstream of Mechanosensing
4. Materials and Methods
4.1. Human Blood Sample
4.2. Erythroblast Cell Culture
4.3. Orbital Shaking to Simulate Mechanical Stress
4.4. Stimulation and Western Blot
4.5. Flow Cytometry and Ca2+ Measurement
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Aglialoro, F.; Abay, A.; Yagci, N.; Rab, M.A.E.; Kaestner, L.; van Wijk, R.; von Lindern, M.; van den Akker, E. Mechanical Stress Induces Ca2+-Dependent Signal Transduction in Erythroblasts and Modulates Erythropoiesis. Int. J. Mol. Sci. 2021, 22, 955. https://doi.org/10.3390/ijms22020955
Aglialoro F, Abay A, Yagci N, Rab MAE, Kaestner L, van Wijk R, von Lindern M, van den Akker E. Mechanical Stress Induces Ca2+-Dependent Signal Transduction in Erythroblasts and Modulates Erythropoiesis. International Journal of Molecular Sciences. 2021; 22(2):955. https://doi.org/10.3390/ijms22020955
Chicago/Turabian StyleAglialoro, Francesca, Asena Abay, Nurcan Yagci, Minke A. E. Rab, Lars Kaestner, Richard van Wijk, Marieke von Lindern, and Emile van den Akker. 2021. "Mechanical Stress Induces Ca2+-Dependent Signal Transduction in Erythroblasts and Modulates Erythropoiesis" International Journal of Molecular Sciences 22, no. 2: 955. https://doi.org/10.3390/ijms22020955