Sulforaphane Causes Cell Cycle Arrest and Apoptosis in Human Glioblastoma U87MG and U373MG Cell Lines under Hypoxic Conditions
Abstract
:1. Introduction
2. Results
2.1. The Effects of SFN on Cell Viability
2.2. The Effects of SFN on Apoptotic Cell Death
2.3. The Effects of SFN on Cell Cycle and Cell Motility
2.4. The Effects of SFN on Mitochondrial and Oxidative Status
2.5. The Effect of SFN on MAPK Signaling Pathway
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatments
4.2. Determination of Cell Viability
4.3. Determination of Apoptotic Cell Death
4.4. Analysis of Cell Cycle
4.5. Determination of Cell Motility
4.6. Determination of GSH Levels
4.7. Determination of Phospho-ERK1/2 and Cytochrome c
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sita, G.; Graziosi, A.; Hrelia, P.; Morroni, F. Sulforaphane Causes Cell Cycle Arrest and Apoptosis in Human Glioblastoma U87MG and U373MG Cell Lines under Hypoxic Conditions. Int. J. Mol. Sci. 2021, 22, 11201. https://doi.org/10.3390/ijms222011201
Sita G, Graziosi A, Hrelia P, Morroni F. Sulforaphane Causes Cell Cycle Arrest and Apoptosis in Human Glioblastoma U87MG and U373MG Cell Lines under Hypoxic Conditions. International Journal of Molecular Sciences. 2021; 22(20):11201. https://doi.org/10.3390/ijms222011201
Chicago/Turabian StyleSita, Giulia, Agnese Graziosi, Patrizia Hrelia, and Fabiana Morroni. 2021. "Sulforaphane Causes Cell Cycle Arrest and Apoptosis in Human Glioblastoma U87MG and U373MG Cell Lines under Hypoxic Conditions" International Journal of Molecular Sciences 22, no. 20: 11201. https://doi.org/10.3390/ijms222011201