Mechanical Properties of Human Concentrated Growth Factor (CGF) Membrane and the CGF Graft with Bone Morphogenetic Protein-2 (BMP-2) onto Periosteum of the Skull of Nude Mice
Abstract
:1. Introduction
2. Results
2.1. Histological and SEM Findings of Concentrated Growth Factor (CGF) Glue
2.2. Initial Length, Width, and Thickness of CGF Membranes
2.3. Tensile Strength and Elastic Moduli
2.4. Concentrated Growth Factor with Recombinant Human Bone Morphogenetic Protein-2 (CGF/BMP-2) Membrane Onto Periosteum of Skull of Head Skins
2.5. Concentrated Growth Factor (CGF) Membrane Alone onto Periosteum of Skull of Head Skins
3. Discussion
4. Materials and Methods
4.1. Preparation of Concentrated Growth Factor (CGF) Glue
4.1.1. Histological Observation of Fresh CGF Glue
4.1.2. Scanning Electron Microscopy (SEM) Observation of Fresh CGF Glue
4.2. Mechanical test of Concentrated Growth Factor (CGF) Membrane
4.2.1. Preparations of Concentrated Growth Factor (CGF) Membrane
4.2.2. Mechanical Properties of Concentrated Growth Factor (CGF) Membrane
4.3. Animal Experiments
4.3.1. Preparation of Concentrated Growth Factor with Recombinant Human Bone Morphogenetic Protein 2 (CGF/BMP-2) Membranes
4.3.2. Ectopic Bioassay (Supra-Periosteal Site)
4.3.3. Tissue Preparation
4.4. Ethical Approval
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
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Day | Length (mm) | Width (mm) | Thickness (mm) |
---|---|---|---|
0 | 27.26 ± 1.38 | 7.27 ± 1.19 | 0.58 ± 0.10 |
1 | 28.92 ± 3.55 | 6.41 ± 0.74 | 0.58 ± 0.09 |
3 | 28.80 ± 1.59 | 7.13 ± 0.64 | 0.43 ± 0.07 |
7 | 26.02 ± 1.89 | 6.89 ± 0.80 | 0.45 ± 0.07 |
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Kabir, M.A.; Hirakawa, A.; Zhu, B.; Yokozeki, K.; Shakya, M.; Huang, B.; Akazawa, T.; Todoh, M.; Murata, M. Mechanical Properties of Human Concentrated Growth Factor (CGF) Membrane and the CGF Graft with Bone Morphogenetic Protein-2 (BMP-2) onto Periosteum of the Skull of Nude Mice. Int. J. Mol. Sci. 2021, 22, 11331. https://doi.org/10.3390/ijms222111331
Kabir MA, Hirakawa A, Zhu B, Yokozeki K, Shakya M, Huang B, Akazawa T, Todoh M, Murata M. Mechanical Properties of Human Concentrated Growth Factor (CGF) Membrane and the CGF Graft with Bone Morphogenetic Protein-2 (BMP-2) onto Periosteum of the Skull of Nude Mice. International Journal of Molecular Sciences. 2021; 22(21):11331. https://doi.org/10.3390/ijms222111331
Chicago/Turabian StyleKabir, Md. Arafat, Akihiro Hirakawa, Bowen Zhu, Kenji Yokozeki, Mamata Shakya, Bingzhen Huang, Toshiyuki Akazawa, Masahiro Todoh, and Masaru Murata. 2021. "Mechanical Properties of Human Concentrated Growth Factor (CGF) Membrane and the CGF Graft with Bone Morphogenetic Protein-2 (BMP-2) onto Periosteum of the Skull of Nude Mice" International Journal of Molecular Sciences 22, no. 21: 11331. https://doi.org/10.3390/ijms222111331