Heterotrimeric G Proteins in Plants: Canonical and Atypical Gα Subunits

Round 1

Reviewer 1 Report

In the present manuscript, the authors present a review highlighting the differences of the heterotrimeric G signaling proteins in plants with other organisms.

The work is well organized and presents the latest developments in this area of knowledge, highlighting the differences in the activation a signaling of plant G proteins with the canonical ones of other eukaryotes.

I recommend to accept this review, with minor corrections indicated in the attached file I the section for authors.

Comments for author File: Comments.pdf

Author Response

Thank you very much for the reviewing our manuscript. Your corrections were addressed. The subtitle and the last paragraph fonts were fixed accordingly.

Reviewer 2 Report

This is nice and sound writing. It reads well and contains an exhaustive information, first of all regarding molecular interactions within plant trimeric G proteins and their protein partners, and notable, sometimes even qualitative, differences between respective mechanisms in plant and animal counterparts. 

There are few suggestions to improve the paper

1. Title: requires rephrasing. Although canonical and non-canonical alpha subunits is central for this review, there is not all only about it, but a substantially wider panorame is considered.  
2.  Line 64. Looks like a subheader, please, take care.
3. Whereas figures 1 and 2 are absolutely the must, I feel that to attract a broader interest an additional figure is lacking, which depicts some physiological function of GPAs and XLGs (e.g. en PAMP perception and transduccion) and hypothetical signaling models. I understand that there are perhaps too many open questions, but in all cases a picture attracts more attention than hundreds of words. 

 

Author Response

Thank you very much for your review. We accepted all your suggestions as follows:

There are few suggestions to improve the paper

1. Title: requires rephrasing. Although canonical and non-canonical alpha subunits is central for this review, there is not all only about it, but a substantially wider panorame is considered.

 

The title was changed as suggested. New title is Heterotrimeric G proteins in plants: canonical and atypical Gα subunits.  

1.  Line 64. Looks like a subheader, please, take care.

The subtitle was fixed accordingly.

1. Whereas figures 1 and 2 are absolutely the must, I feel that to attract a broader interest an additional figure is lacking, which depicts some physiological function of GPAs and XLGs (e.g. en PAMP perception and transduccion) and hypothetical signaling models. I understand that there are perhaps too many open questions, but in all cases a picture attracts more attention than hundreds of words. 

 A new figure was made and added to the manuscript. The manuscript with the new figure is attached.

Author Response File: Author Response.docx